ArticleBMC plant biology2011
High resolution melting analysis for the detection of EMS induced mutations in wheat SBEIIa genes.
Article in BMC plant biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Metabolic response to amylose-rich wheat-based rusks in overweight individuals.European journal of clinical nutrition · 2018Trial
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- The impact of GBSSI inactivation on starch structure and functionality in EMS-induced mutant lines of wheat.BMC genomics · 2025Article
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat.Frontiers in genome editing · 2025Article
- Stacked mutations in wheat homologues of rice SEMI-DWARF1 confer a novel semi-dwarf phenotype.BMC plant biology · 2024Article
- Comparison of genotyping assays for detection of targeted CRISPR/Cas mutagenesis in highly polyploid sugarcane.Frontiers in genome editing · 2024Article
- Detection of genetic divergence among putative ethyl methane sulfonate mutants of super basmati using microsatellite markers.Molecular biology reports · 2023Article
- Boosting wheat functional genomics via an indexed EMS mutant library of KN9204.Plant communications · 2023Article
- Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis.Planta · 2023Review
- The suppression of TdMRP3 genes reduces the phytic acid and increases the nutrient accumulation in durum wheat grain.Frontiers in plant science · 2023Article
- Regulation of Amylose Content by Single Mutations at an Active Site in the Wx-B1 Gene in a Tetraploid Wheat Mutant.International journal of molecular sciences · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Increasing the Versatility of Durum Wheat through Modifications of Protein and Starch Composition and Grain Hardness.Foods (Basel, Switzerland) · 2022Review
- Effects of the different waxy proteins on starch biosynthesis, starch physicochemical properties and Chinese noodle quality in wheat.Molecular breeding : new strategies in plant improvement · 2022Article
- Article
- Deficiency of a peroxisomal NADP-isocitrate dehydrogenase leads to dwarf plant and defect seed in upland cotton.Frontiers in plant science · 2022Article
- Enrichment of provitamin A content in durum wheat grain by suppressing β-carotene hydroxylase 1 genes with a TILLING approach.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.BMC plant biology · 2021Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundManipulation of the amylose-amylopectin ratio in cereal starch has been identified as a major target for the production of starches with novel functional properties. In wheat, silencing of starch branching enzyme genes by a transgenic approach reportedly caused an increase of amylose content up to 70% of total starch, exhibiting novel and interesting nutritional characteristics. In this work, the functionality of starch branching enzyme IIa (SBEIIa) has been targeted in bread wheat by TILLING. An EMS-mutagenised wheat population has been screened using High Resolution Melting of PCR products to identify functional SNPs in the three homoeologous genes encoding the target enzyme in the hexaploid genome.
resultsThis analysis resulted in the identification of 56, 14 and 53 new allelic variants respectively for SBEIIa-A, SBEIIa-B and SBEIIa-D. The effects of the mutations on protein structure and functionality were evaluated by a bioinformatic approach. Two putative null alleles containing non-sense or splice site mutations were identified for each of the three homoeologous SBEIIa genes; qRT-PCR analysis showed a significant decrease of their gene expression and resulted in increased amylose content. Pyramiding of different single null homoeologous allowed to isolate double null mutants showing an increase of amylose content up to 21% compared to the control.
conclusionTILLING has successfully been used to generate novel alleles for SBEIIa genes known to control amylose content in wheat. Single and double null SBEIIa genotypes have been found to show a significant increase in amylose content.
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