ArticleEuropean journal of histochemistry : EJH2009
Localization of DNA methyltransferase-1 during oocyte differentiation, in vitro maturation and early embryonic development in cow.
Article in European journal of histochemistry : EJH, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- DNA Methylation in the Ovary and Uterus of Mammalian Animal Models: Implications for Reproductive Function.Genes · 2026Review
- Approaches toAnimal reproduction · 2025Article
- Pramel15 facilitates zygotic nuclear DNMT1 degradation and DNA demethylation.Nature communications · 2024Article
- Expression profile of viability and stress response genes as a result of resveratrol supplementation in vitrified and in vitro produced cattle embryos.Molecular biology reports · 2024Article
- Dynamic methylation pattern of H19DMR and KvDMR1 in bovine oocytes and preimplantation embryos.Journal of assisted reproduction and genetics · 2024Article
- Differentially Expressed Candidate miRNAs of Day 16 Bovine Embryos on the Regulation of Pregnancy Establishment in Dairy Cows.Animals : an open access journal from MDPI · 2023Article
- Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Impact of nano-selenium on nuclear maturation and genes expression profile of buffalo oocytes matured in vitro.Molecular biology reports · 2020Article
- DNA methylation changes during preimplantation development reveal inter-species differences and reprogramming events at imprinted genes.Clinical epigenetics · 2020Article
- DNMT1, DNMT3A and DNMT3B proteins are differently expressed in mouse oocytes and early embryos.Journal of molecular histology · 2017Article
- A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs.PloS one · 2015Article
- Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte.Journal of assisted reproduction and genetics · 2013Article
- Large-scale chromatin morpho-functional changes during mammalian oocyte growth and differentiation.European journal of histochemistry : EJH · 2012Review
- SIRT1 deacetylates the DNA methyltransferase 1 (DNMT1) protein and alters its activities.Molecular and cellular biology · 2011Article
- DNA methyl transferase 1: regulatory mechanisms and implications in health and disease.International journal of biochemistry and molecular biology · 2011Article
- Histochemistry through the years, browsing a long-established journal: novelties in traditional subjects.European journal of histochemistry : EJH · 2010Article
- The variable success of in vitro maturation: can we do better?Animal reproductionArticle
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Authors and funding
6 authors.
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Abstract
DNA methyltransferase-1 (Dnmt1) is involved in the maintenance of DNA methylation patterns and is crucial for normal mammalian development. The aim of the present study was to assess the localization of Dnmt1 in cow, during the latest phases of oocyte differentiation and during the early stages of segmentation. Dnmt1 expression and localization were assessed in oocytes according to the chromatin configuration, which in turn provides an important epigenetic mechanism for the control of global gene expression and represents a morphological marker of oocyte differentiation. We found that the initial chromatin condensation was accompanied by a slight increase in the level of global DNA methylation, as assessed by 5-methyl-cytosine immunostaining followed by laser scanning confocal microscopy analysis (LSCM). RT-PCR confirmed the presence of Dnmt1 transcripts throughout this phase of oocyte differentiation. Analogously, Dnmt1 immunodetection and LSCM indicated that the protein was always present and localized in the cytoplasm, regardless the chromatin configuration and the level of global DNA methylation. Moreover, our data indicate that while Dnmt1 is retained in the cytoplasm in metaphase II stage oocytes and zygotes, it enters the nuclei of 8-16 cell stage embryos. As suggested in mouse, the functional meaning of the presence of Dnmt1 in the bovine embryo nuclei could be the maintainement of the methylation pattern of imprinted genes. In conclusion, the present work provides useful elements for the study of Dnmt1 function during the late stage of oocyte differentiation, maturation and early embryonic development in mammals.
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