ArticlePloS one2011
Use of a high resolution melting (HRM) assay to compare gag, pol, and env diversity in adults with different stages of HIV infection.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 37 citations in OpenAlex.
- Association of HIV diversity and virologic outcomes in early antiretroviral treatment: HPTN 052.PloS one · 2017Trial
- Use of a multifaceted approach to analyze HIV incidence in a cohort study of women in the United States: HIV Prevention Trials Network 064 Study.The Journal of infectious diseases · 2013Trial
- Association of pol diversity with antiretroviral treatment outcomes among HIV-infected African children.PloS one · 2013Trial
- HIV-1 amino acid changes among participants with virologic failure: associations with first-line efavirenz or atazanavir plus ritonavir and disease status.The Journal of infectious diseases · 2012Trial
- Recent HIV Infection: Diagnosis and Public Health Implications.Diagnostics (Basel, Switzerland) · 2022Review
- Statistical considerations for cross-sectional HIV incidence estimation based on recency test.Statistics in medicine · 2022Article
- Modeling methods for estimating HIV incidence: a mathematical review.Theoretical biology & medical modelling · 2020Review
- Performance characteristics of an antibody-based multiplex kit for determining recent HIV-1 infection.PloS one · 2017Article
- Article
- Strengthening HIV surveillance: measurements to track the epidemic in real time.African journal of AIDS research : AJAR · 2016Article
- Analysis of HIV Diversity in HIV-Infected Black Men Who Have Sex with Men (HPTN 061).PloS one · 2016Article
- Analysis of Viral Diversity in Relation to the Recency of HIV-1C Infection in Botswana.PloS one · 2016Article
- Review
- A Generalized Entropy Measure of Within-Host Viral Diversity for Identifying Recent HIV-1 Infections.Medicine · 2015Article
- How can we better identify early HIV infections?Current opinion in HIV and AIDS · 2015Review
- HIV diversity as a biomarker for HIV incidence estimation: including a high-resolution melting diversity assay in a multiassay algorithm.Journal of clinical microbiology · 2014Article
- A comparison of two measures of HIV diversity in multi-assay algorithms for HIV incidence estimation.PloS one · 2014Article
- Cross-sectional HIV incidence estimation in HIV prevention research.Journal of acquired immune deficiency syndromes (1999) · 2013Article
- Future of phylogeny in HIV prevention.Journal of acquired immune deficiency syndromes (1999) · 2013Article
- HIV incidence determination in the United States: a multiassay approach.The Journal of infectious diseases · 2013Article
Corrections and comments
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Authors and funding
18 authors at 8 institutions in 1 country.
Funding
Abstract
backgroundCross-sectional assessment of HIV incidence relies on laboratory methods to discriminate between recent and non-recent HIV infection. Because HIV diversifies over time in infected individuals, HIV diversity may serve as a biomarker for assessing HIV incidence. We used a high resolution melting (HRM) diversity assay to compare HIV diversity in adults with different stages of HIV infection. This assay provides a single numeric HRM score that reflects the level of genetic diversity of HIV in a sample from an infected individual.
methodsHIV diversity was measured in 203 adults: 20 with acute HIV infection (RNA positive, antibody negative), 116 with recent HIV infection (tested a median of 189 days after a previous negative HIV test, range 14-540 days), and 67 with non-recent HIV infection (HIV infected >2 years). HRM scores were generated for two regions in gag, one region in pol, and three regions in env.
resultsMedian HRM scores were higher in non-recent infection than in recent infection for all six regions tested. In multivariate models, higher HRM scores in three of the six regions were independently associated with non-recent HIV infection.
conclusionsThe HRM diversity assay provides a simple, scalable method for measuring HIV diversity. HRM scores, which reflect the genetic diversity in a viral population, may be useful biomarkers for evaluation of HIV incidence, particularly if multiple regions of the HIV genome are examined.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.