ArticleBiophysical journal1990
Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes.
Article in Biophysical journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)-Cell Interaction and the Resultant Bioeffects at the Single-cell Level.Journal of visualized experiments : JoVE · 2017Article
- Cell membrane deformation and bioeffects produced by tandem bubble-induced jetting flow.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Biomechanics: cell research and applications for the next decade.Annals of biomedical engineering · 2009Article
- Mapping mechanical strain of an endogenous cytoskeletal network in living endothelial cells.Biophysical journal · 2003Article
- Leukocyte biophysics. An invited review.Cell biophysics · 1990Review
- Strain measurements in cultured vascular smooth muscle cells subjected to mechanical deformation.Annals of biomedical engineeringArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A new method is presented to measure local cytoplasmic deformation and rate of deformation in motile active neutrophils. The deformation is expressed in terms of biomechanical strains and strain rates. For this purpose small phagocytosed latex microspheres were used as intracellular markers. Planar Lagrangian and Eulerian strains and the rate of strain were estimated from the positions of a triad of internalized markers. Principal strains, stretch ratios, and principal directions were computed. The intracellular strains were found to be large relative to the overall cell shape change. Principal cytoplasmic stretch ratios showed large extension in the direction of pseudopod formation and cell locomotion and contraction in perpendicular directions. Regional strain analysis showed contractile strains to predominate in the vicinity of the pseudopod or leading edge of motion. The transitional region between the pseudopod and the main cell body exhibited large shear strains. The posterior region, where the uropod is located, also revealed large extensions but small contractile strains. The rate of strains are relatively small, nonuniform in time, and largely independent of the strain. The method we propose to measure cytoplasmic strain can be applied to a variety of problems in cell mechanics.
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Registered trials
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