ArticleToxins2011
Loss of vtx genes after the first subcultivation step of verocytotoxigenic Escherichia coli O157 and Non-O157 during isolation from naturally contaminated fecal samples.
Article in Toxins, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Genetic variability of Shiga toxin-producingMicrobiology spectrum · 2025Article
- Prevalence of Shiga toxin-producing Escherichia Coli O157 in Moroccan food and antimicrobial resistance profiles of the strains isolated.World journal of microbiology & biotechnology · 2025Article
- The Trade-Off Between Sanitizer Resistance and Virulence Genes: Genomic Insights intoAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Wild-Type Domestication: Loss of Intrinsic Metabolic Traits Concealed by Culture in Rich Media.Microbial ecology · 2024Article
- Evidence for Horizontal Transmission and Recirculation of Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2024Article
- Influence of temperature and pH on induction of Shiga toxinFrontiers in microbiology · 2023Article
- Prevalence and Characterization of Shiga Toxin ProducingMicroorganisms · 2022Article
- Inconsistent PCR detection of Shiga toxin-producing Escherichia coli: Insights from whole genome sequence analyses.PloS one · 2021Article
- Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups.Microbial ecology · 2021Article
- Characterization of Non-O157Microorganisms · 2019Article
- Prevalence and concentration of stx+ E. coli and E. coli O157 in bovine manure from Florida farms.PloS one · 2019Article
- Implications of stx loss for clinical diagnostics of Shiga toxin-producing Escherichia coli.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Variability in CharacterizingMicroorganisms · 2018Article
- Evidence for Contemporary Switching of the O-Antigen Gene Cluster between Shiga Toxin-ProducingFrontiers in microbiology · 2017Article
- The Accessory Genome of Shiga Toxin-Producing Escherichia coli Defines a Persistent Colonization Type in Cattle.Applied and environmental microbiology · 2016Article
- Characterization of Shiga Toxin Subtypes and Virulence Genes in Porcine Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Article
- Risk Factors for Salmonella, Shiga Toxin-Producing Escherichia coli and Campylobacter Occurrence in Primary Production of Leafy Greens and Strawberries.International journal of environmental research and public health · 2015Article
- Microbial safety and sanitary quality of strawberry primary production in Belgium: risk factors for Salmonella and Shiga toxin-producing Escherichia coli contamination.Applied and environmental microbiology · 2015Article
- Intimin gene (eae) subtype-based real-time PCR strategy for specific detection of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 in cattle feces.Applied and environmental microbiology · 2014Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Verocytotoxins VT1 and VT2,produced by Verocytotoxigenic Escherichia coli (VTEC), are encoded on temperate bacteriophages. Several studies reported the loss of the vtx genes after multiple subcultivation steps or long preservation. The objective of this study was to determine if the loss of the verocytotoxin genes can already occur during the first subcultivation step. Consequently, the stability of the vtx genes were tested in 40 isolates originating from 40 vtx-positive fecal samples after the first subcultivation step following the isolation procedure. The loss occurred in 12 out of 40 strains tested and was rather rare among the O157 strains compared to the non-O157 strains. This is the first study demonstrating that the loss of the verocytotoxin genes can already occur after the first subcultivation step. This may lead to an underestimation of VTEC positive samples.
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