ArticleToxins2011
Control of aflatoxin production of Aspergillus flavus and Aspergillus parasiticus using RNA silencing technology by targeting aflD (nor-1) gene.
Article in Toxins, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 64 citations in OpenAlex.
- Space-Confined Hydrogel Particle-Based Biosensor for Early Warning of Aflatoxin B1 via Rapid Label-Free Fluorescence Detection of theAnalytical chemistry · 2025Article
- RNA-mediated gene silencing can reduce azole resistance, growth and pathogenicity in Pseudocercospora fijiensis.PloS one · 2025Article
- RNAi-Based Approaches to Control Mycotoxin Producers: Challenges and Perspectives.Journal of fungi (Basel, Switzerland) · 2024Review
- Disruption of theRNA (New York, N.Y.) · 2023Article
- Multiplexed Host-Induced Gene Silencing ofToxins · 2023Article
- Advanced mycotoxin control and decontamination techniques in view of an increased aflatoxin risk in Europe due to climate change.Frontiers in microbiology · 2022Review
- Biodiversity of AflatoxigenicFrontiers in microbiology · 2020Article
- Development of an RNA interference (RNAi) gene knockdown protocol in the anaerobic gut fungusPeerJ · 2018Article
- RNAi-mediated down-regulation of a melanin polyketide synthase (pks1) gene in the fungus Slafractonia leguminicola.World journal of microbiology & biotechnology · 2017Article
- The Utilization of RNA Silencing Technology to Mitigate the Voriconazole Resistance of Aspergillus Flavus; Lipofectamine-Based Delivery.Advanced pharmaceutical bulletin · 2017Article
- RNA Interference (RNAi) as a Potential Tool for Control of Mycotoxin Contamination in Crop Plants: Concepts and Considerations.Frontiers in plant science · 2017Review
- Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae.BMC biotechnology · 2016Article
- Effects of Nutrients in Substrates of Different Grains on Aflatoxin B1 Production by Aspergillus flavus.BioMed research international · 2016Article
- Adenylate Cyclase AcyA Regulates Development, Aflatoxin Biosynthesis and Fungal Virulence inFrontiers in cellular and infection microbiology · 2016Article
- RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem.Journal of visualized experiments : JoVE · 2015Article
- Downregulation of transcription factor aflR in Aspergillus flavus confers reduction to aflatoxin accumulation in transgenic maize with alteration of host plant architecture.Plant cell reports · 2015Article
- Characterization of RNA silencing components in the plant pathogenic fungus Fusarium graminearum.Scientific reports · 2015Article
- Integrating toxin gene expression, growth and fumonisin B1 and B2 production by a strain of Fusarium verticillioides under different environmental factors.Journal of the Royal Society, Interface · 2013Article
- Host-induced gene silencing: a tool for understanding fungal host interaction and for developing novel disease control strategies.Molecular plant pathology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus flavus and Aspergillus parasiticus are important pathogens of cotton, corn, peanuts and other oil-seed crops, producing toxins both in the field and during storage. We have designed three siRNA sequences (Nor-Ia, Nor-Ib, Nor-Ic) to target the mRNA sequence of the aflD gene to examine the potential for using RNA silencing technology to control aflatoxin production. Thus, the effect of siRNAs targeting of two key genes in the aflatoxin biosynthetic pathway, aflD (structural) and aflR (regulatory gene) and on aflatoxin B(1 )(AFB(1)), and aflatoxin G(1) (AFG(1)) production was examined. The study showed that Nor-Ib gave a significant decrease in aflD mRNA, aflR mRNA abundance, and AFB(1) production (98, 97 and 97% when compared to the controls) in A. flavus NRRL3357, respectively. Reduction in aflD and aflR mRNA abundance and AFB(1 )production increased with concentration of siRNA tested. There was a significant inhibition in aflD and AFB(1) production by A. flavus EGP9 and AFG(1 )production by A. parasiticus NRRL 13005. However, there was no significant decrease in AFG(1) production by A. parasiticus SSWT 2999. Changes in AFB(1) production in relation to mRNA levels of aflD showed a good correlation (R = 0.88; P = 0.00001); changes in aflR mRNA level in relation to mRNA level of aflD also showed good correlation (R = 0.82; P = 0.0001). The correlations between changes in aflR and aflD gene expression suggests a strong relationship between these structural and regulatory genes, and that aflD could be used as a target gene to develop efficient means for aflatoxin control using RNA silencing technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.