ArticlePloS one2011
Mll5 is required for normal spermatogenesis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- KMT2E recruitment by H3 serotonylation regulates neurodevelopmental chromatin dynamics.bioRxiv : the preprint server for biology · 2026Article
- SETD5 regulates the OGT-catalyzed O-GlcNAcylation of RNA polymerase II, which is involved in the stemness of colorectal cancer cells.Scientific reports · 2023Article
- Actin-related protein ACTL7B ablation leads to OAT with multiple morphological abnormalities of the flagellum and male infertility in mice†.Biology of reproduction · 2023Article
- A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm.Journal of assisted reproduction and genetics · 2021Article
- Case Report:Frontiers in pediatrics · 2021Article
- MLL5, a histone modifying enzyme, regulates androgen receptor activity in prostate cancer cells by recruiting co-regulators, HCF1 and SET1.BMB reports · 2020Article
- Genomic Signatures of Selection Associated With Litter Size Trait in Jining Gray Goat.Frontiers in genetics · 2020Article
- Heterozygous Variants in KMT2E Cause a Spectrum of Neurodevelopmental Disorders and Epilepsy.American journal of human genetics · 2019Article
- SET domains and stress: uncovering new functions for yeast Set4.Current genetics · 2019Review
- Effect of Early Calf-Hood Nutrition on the Transcriptional Regulation of the Hypothalamic-Pituitary-Testicular axis in Holstein-Friesian Bull Calves.Scientific reports · 2018Article
- Insertion/Deletion Within the KDM6A Gene Is Significantly Associated With Litter Size in Goat.Frontiers in genetics · 2018Article
- Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.PloS one · 2018Article
- MLL5 (KMT2E): structure, function, and clinical relevance.Cellular and molecular life sciences : CMLS · 2017Review
- Setd5 is essential for mammalian development and the co-transcriptional regulation of histone acetylation.Development (Cambridge, England) · 2016Article
- MLL5 maintains spindle bipolarity by preventing aberrant cytosolic aggregation of PLK1.The Journal of cell biology · 2016Article
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- Association of the histone-lysine N-methyltransferase MLL5 gene with coronary artery disease in Chinese Han people.Meta gene · 2014Article
- Purification, crystallization and preliminary X-ray diffraction analysis of the kinase domain of human tousled-like kinase 2.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Histone H3 lysine 4 methyltransferases and demethylases in self-renewal and differentiation of stem cells.Cell & bioscience · 2013Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundMll5 is currently a member of the Mll family of SET domain histone methyltransferase proteins but studies have also showed that it could be part of the SET3 branch of proteins. Recently, constitutive knock out animal studies have shown that Mll5 is required for proper haematopoietic stem cell differentiation, and loss of Mll5 results in synthetic lethality for genome de-methylation. Mll5 deficient male mice are infertile and here we analyse the consequences of Mll5 deficiency for spermatogenesis. METHODOLOGY/PRINCIPAL
findingsMll5 deficient male mice, but not female mice, are infertile. Here we show using RNA in-situ hybridization that Mll5 is expressed in the germ cells of the testes of wild type mice. Consistent with the expression of Mll5, we demonstrate by electron microscopy, video microscopy and in vitro fertilisation techniques that Mll5 deficient mice have defects in terminal maturation and packaging of sperm. The defects seen include detachment of the acrosomal cap and impaired excess cytoplasm removal. Functional tests of sperm motility show a lack of progressive motility of spermatozoa from Mll5 deficient animals. None of these defects could be rescued by in vitro fertilization. Using microarray analysis we show that transcripts implicated in spermatogenesis are dysregulated. CONCLUSIONS/SIGNIFICANCE: Our data demonstrate a clear role of Mll5 in mammalian spermatogenesis at the level of terminal differentiation providing further support for its classification in the SET3 branch of proteins. Moreover, this study identifies Tlk2, Utx, Gpr64, Sult4a1, Rap2ip, Vstm2 and HoxA10 as possible Mll5 targets that together may account for the observed spermatozoa maturation defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.