ArticlePloS one2011
A single nucleotide in stem loop II of 5'-untranslated region contributes to virulence of enterovirus 71 in mice.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 81 citations in OpenAlex.
- Physicochemical fingerprinting reveals convergent evolutionary determinants of enterovirus A71 neurovirulence through integrative machine learning and structural analysis.Virus research · 2026Article
- Article
- Review
- Novel reporter constructs to accelerate antiviral and therapeutic discovery for Enterovirus-A71.Antiviral research · 2025Article
- Deep mutation, insertion and deletion scanning across the Enterovirus A proteome reveals constraints shaping viral evolution.Nature microbiology · 2025Article
- Enterovirus D68: Genomic and Clinical Comparison of 2 Seasons of Increased Viral Circulation and Discrepant Incidence of Acute Flaccid Myelitis-Maryland, USA.Open forum infectious diseases · 2024Article
- EV71 5'UTR interacts with 3D protein affecting replication through the AKT-mTOR pathway.Virology journal · 2024Article
- Uncovering Structural Plasticity ofResearch square · 2024Article
- A tradeoff between enterovirus A71 particle stability and cell entry.Nature communications · 2023Article
- Clinical and molecular epidemiologic features of enterovirus D68 infection in children with acute lower respiratory tract infection in China.Archives of virology · 2023Review
- Role of RNA Domain Structure and Orientation in the Coxsackievirus B3 Virulence Phenotype.Journal of virology · 2023Article
- Insights into In Vitro Adaptation of EV71 and Analysis of Reduced Virulence by In Silico Predictions.Vaccines · 2023Article
- Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses.Microbiology spectrum · 2023Article
- RNA Structure in the 5' Untranslated Region of Enterovirus D68 Strains with Differing Neurovirulence Phenotypes.Viruses · 2023Article
- Viral determinants that drive Enterovirus-A71 fitness and virulence.Emerging microbes & infections · 2021Review
- Enterovirus A71 Vaccines.Vaccines · 2021Review
- Mapping Attenuation Determinants in Enterovirus-D68.Viruses · 2020Article
- Characterization of Plaque Variants and the Involvement of Quasi-Species in a Population of EV-A71.Viruses · 2020Article
- Heparan sulfate attachment receptor is a major selection factor for attenuated enterovirus 71 mutants during cell culture adaptation.PLoS pathogens · 2020Article
- Cellular receptors for enterovirus A71.Journal of biomedical science · 2020Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnterovirus 71 (EV71) has emerged as a neuroinvasive virus responsible for several large outbreaks in the Asia-Pacific region while virulence determinant remains unexplored. PRINCIPAL
findingsIn this report, we investigated increased virulence of unadapted EV71 clinical isolate 237 as compared with isolate 4643 in mice. A fragment 12 nucleotides in length in stem loop (SL) II of 237 5'-untranslated region (UTR) visibly reduced survival time and rate in mice was identified by constructing a series of infectious clones harboring chimeric 5'-UTR. In cells transfected with bicistronic plasmids, and replicon RNAs, the 12-nt fragment of isolate 237 enhanced translational activities and accelerated replication of subgenomic EV71. Finally, single nucleotide change from cytosine to uridine at base 158 in this short fragment of 5'-UTR was proven to reduce viral translation and EV71 virulence in mice. Results collectively indicated a pivotal role of novel virulence determinant C158 on virus translation in vitro and EV71 virulence in vivo.
conclusionsThese results presented the first reported virulence determinant in EV71 5'-UTR and first position discovered from unadapted isolates.
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