ArticleThe Journal of biological chemistry2011
Functional analysis of C-type lysozyme in penaeid shrimp.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 82 citations in OpenAlex.
- Feedback coordination of FoxO-mediated antibacterial immunity by PDGF/VEGF signaling establishes hemolymph microbiota homeostasis in shrimp.PLoS pathogens · 2026Article
- Rethinking Disease Control in Aquaculture Invertebrates: Harnessing Innate Immunity in Molluscs and Crustaceans.Pathogens (Basel, Switzerland) · 2026Review
- AnMethodsX · 2023Article
- Taxifolin Inhibits WSSV Infection and Transmission by Increasing the Innate Immune Response inViruses · 2022Article
- Small immune effectors coordinate peptidoglycan-derived immunity to regulate intestinal bacteria in shrimp.PLoS pathogens · 2022Article
- Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of Whiteleg Shrimp (Aquaculture nutrition · 2022Article
- Integrated analysis of intestinal microbiota and metabolomic reveals that decapod iridescent virus 1 (DIV1) infection induces secondary bacterial infection and metabolic reprogramming inFrontiers in immunology · 2022Article
- Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic.Antibiotics (Basel, Switzerland) · 2021Review
- Identification and characterization of two highly homologous lysozymes from red swamp crayfish,Fish and shellfish immunology reports · 2021Article
- The Molecular Mechanism of Hemocyte Immune Response inFrontiers in microbiology · 2021Article
- Two Distinct C-Type Lysozymes in Goldfish: Molecular Characterization, Antimicrobial Potential, and Transcriptional Regulation in Response to Opposing Effects of Bacteria/Lipopolysaccharide and Dexamethasone/Leptin.International journal of molecular sciences · 2020Article
- The Two NF-κB Pathways Regulating Bacterial and WSSV Infection of Shrimp.Frontiers in immunology · 2019Review
- Hemolymph Microbiomes of Three Aquatic Invertebrates as Revealed by a New Cell Extraction Method.Applied and environmental microbiology · 2018Article
- Functional Characterization of a c-type Lysozyme from Indian Shrimp Fenneropenaeus indicus.Probiotics and antimicrobial proteins · 2014Article
- A shrimp C-type lectin inhibits proliferation of the hemolymph microbiota by maintaining the expression of antimicrobial peptides.The Journal of biological chemistry · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lysozyme is an enzyme that cleaves the β-1,4-glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine in peptidoglycan, leading to bacterial lysis. Recently, lysozyme has been found to have anti-HIV and anti-cancer properties in mammals. However, most functional analyses were done in vitro using purified or recombinant lysozyme protein. Here, we used RNA interference to silence c-type lysozyme expression in penaeid shrimp, Marsupenaeus japonicus, to analyze the function of lysozyme in vivo. Silencing of lysozyme expression by dsRNA lysozyme (dsLYZ) led to 100% mortality without any artificial bacterial infection in 5 days. Lysozyme deficiency caused the number of hemocytes in hemolymph to decrease from 1.3 × 10(7) to 2.3 × 10(6) cells/ml and caused the number of bacteria to increase from 78 to 764 colony-forming units/ml. Suppression of bacterial growth using oxytetracycline and kanamycin showed improvement in mortality, suggesting that shrimp mortality post- dsLYZ injection can be attributed to bacterial growth in the shrimp hemolymph. The majority of the bacteria, identified by 16 S rRNA analysis, were Gram-negative species such as Vibrio and Pseudomonas. Furthermore, PKH26 staining showed that the dsLYZ-injected shrimp were unable to eliminate non pathogenic Escherichia coli or Staphylococcus aureus in 24 h. These data suggest that c-type lysozyme in shrimp serves to regulate the growth of bacterial communities, particularly Gram-negative bacteria, in the hemolymph.
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