ArticleEuropean biophysics journal : EBJ2012
A method for analysis of lipid vesicle domain structure from confocal image data.
Article in European biophysics journal : EBJ, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Movement of giant lipid vesicles induced by millimeter wave radiation change when they contain magnetic nanoparticles.Drug delivery and translational research · 2019Article
- Capturing suboptical dynamic structures in lipid bilayer patches formed from free-standing giant unilamellar vesicles.Nature protocols · 2017Article
- Morphometric image analysis of giant vesicles: a new tool for quantitative thermodynamics studies of phase separation in lipid membranes.Biophysical journal · 2012Article
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Authors and funding
5 authors.
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Abstract
Quantitative characterization of the lateral structure of curved membranes based on fluorescence microscopy requires knowledge of the fluorophore distribution on the surface. We present an image analysis approach for extraction of the fluorophore distribution on a spherical lipid vesicle from confocal imaging stacks. The technique involves projection of volumetric image data onto a triangulated surface mesh representation of the membrane, correction of photoselection effects and global motion of the vesicle during image acquisition and segmentation of the surface into domains using histograms. The analysis allows for investigation of the morphology and size distribution of domains on the surface.
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Registered trials
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