ArticleJournal of structural biology2012
Metallothionein as a clonable high-density marker for cryo-electron microscopy.
Article in Journal of structural biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Molecular tags for electron cryo-tomography.Emerging topics in life sciences · 2025Review
- Genetically encoded barcodes for correlative volume electron microscopy.Nature biotechnology · 2023Article
- Charting the molecular landscape of the cell.Structure (London, England : 1993) · 2023Review
- Challenges and triumphs in cryo-electron tomography.iScience · 2021Review
- Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells.Nature methods · 2020Article
- Glucose starvation triggers filamentous septin assemblies in anBiology open · 2019Article
- Locating macromolecules and determining structures inside bacterial cells using electron cryotomography.Biochimica et biophysica acta. Proteins and proteomics · 2018Review
- Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast.Bio-protocol · 2018Article
- A new view into prokaryotic cell biology from electron cryotomography.Nature reviews. Microbiology · 2016Review
- Metallothionein as a clonable tag for protein localization by electron microscopy of cells.Journal of microscopy · 2015Article
- Lessons from yeast: the spindle pole body and the centrosome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity.Ultramicroscopy · 2014Article
- High-resolution cryo-electron microscopy on macromolecular complexes and cell organelles.Protoplasma · 2014Review
- Stable micron-scale holes are a general feature of canonical holins.Molecular microbiology · 2014Article
- Golgi apparatus analyzed by cryo-electron microscopy.Histochemistry and cell biology · 2013Review
- Cryo-electron microscopy--a primer for the non-microscopist.The FEBS journal · 2013Review
- Specific, sensitive, high-resolution detection of protein molecules in eukaryotic cells using metal-tagging transmission electron microscopy.Structure (London, England : 1993) · 2012Article
- Structural characterization of the complex of SecB and metallothionein-labeled proOmpA by cryo-electron microscopy.PloS one · 2012Article
- A detailed, hierarchical study of Giardia lamblia's ventral disc reveals novel microtubule-associated protein complexes.PloS one · 2012Article
- Picking faces out of a crowd: genetic labels for identification of proteins in correlated light and electron microscopy imaging.Methods in cell biology · 2012Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cryo-electron microscopy is expanding its scope from macromolecules towards much larger and more complex cellular specimens such as organelles, cells and entire tissues. While isolated macromolecular specimens are typically composed of only very few different components that may be recognized by their shape, size or state of polymerization, cellular specimens combine large numbers of proteinaceous structures as well as nucleic acids and lipid arrays. Consequently, an unambiguous identification of these structures within the context of a whole cell may create a very difficult challenge. On plastic-embedded specimens, or Tokuyasu sections (Tokuyasu, 1980), epitopes that are exposed at the surface can be tagged by antibodies. However, vitrified sections have to be kept at strict cryo-conditions (below -140 °C) and therefore do not allow any post-sectioning treatment of the specimens other than data acquisition in the microscope. Hence, the labels have to be placed into the specimen before freezing. Here we report on the application of a small metal-clustering protein, metallothionein (MTH), as a clonable label capable of clustering metal atoms into a high-density particle with high spatial resolution. We tested MTH as a label for kinesin-decorated microtubules (MTs) as well as the building blocks of desmin intermediate filaments (IFs).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.