ArticleMicrobial cell factories2011
A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea.
Article in Microbial cell factories, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Cloning of cellulase gene using metagenomic approach of soils collected from Wadi El Natrun, an extremophilic desert valley in Egypt.Journal, genetic engineering & biotechnology · 2022Article
- Metagenomic Screening for Lipolytic Genes Reveals an Ecology-Clustered Distribution Pattern.Frontiers in microbiology · 2022Article
- Marine Bacterial Esterases: Emerging Biocatalysts for Industrial Applications.Applied biochemistry and biotechnology · 2021Review
- Article
- Improved production of recombinantBioengineered · 2020Article
- Identification and characterization of a novel alkalistable and salt-tolerant esterase from the deep-sea hydrothermal vent of the East Pacific Rise.MicrobiologyOpen · 2018Article
- Characterization of ML-005, a Novel Metaproteomics-Derived Esterase.Frontiers in microbiology · 2018Article
- Identification and Characterization of a Novel Salt-Tolerant Esterase from the Deep-Sea Sediment of the South China Sea.Frontiers in microbiology · 2017Article
- Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases.Scientific reports · 2016Article
- A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters.Microbial cell factories · 2016Article
- Isolation and Characterization of a Novel Cold-Adapted Esterase, MtEst45, from Microbulbifer thermotolerans DAU221.Frontiers in microbiology · 2016Article
- Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome.PloS one · 2016Article
- EstDZ3: A New Esterolytic Enzyme Exhibiting Remarkable Thermostability.Frontiers in microbiology · 2016Article
- Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library.Journal of industrial microbiology & biotechnology · 2015Article
- pSW2, a Novel Low-Temperature-Inducible Gene Expression Vector Based on a Filamentous Phage of the Deep-Sea Bacterium Shewanella piezotolerans WP3.Applied and environmental microbiology · 2015Article
- Characterization of a novel thermostable patatin-like protein from a Guaymas basin metagenomic library.Extremophiles : life under extreme conditions · 2015Article
- The use of fosmid metagenomic libraries in preliminary screening for various biological activities.Microbial cell factories · 2014Article
- Characterization of a novel cold-active esterase isolated from swamp sediment metagenome.World journal of microbiology & biotechnology · 2014Article
- Isolation of a novel alkaline-stable lipase from a metagenomic library and its specific application for milkfat flavor production.Microbial cell factories · 2014Article
- Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel.Biotechnology for biofuels · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMarine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential source of novel enzymes with unique properties that may be useful in industry and biotechnology. To explore the lipolytic genetic resources in the South China Sea, 23 sediment samples were collected in the depth < 100 m marine areas.
resultsA metagenomic library of South China Sea sediments assemblage in plasmid vector containing about 194 Mb of community DNA was prepared. Screening of a part of the unamplified library resulted in isolation of 15 unique lipolytic clones with the ability to hydrolyze tributyrin. A positive recombinant clone (pNLE1), containing a novel esterase (Est_p1), was successfully expressed in E. coli and purified. In a series of assays, Est_p1 displayed maximal activity at pH 8.57, 40°C, with ρ-Nitrophenyl butyrate (C4) as substrate. Compared to other metagenomic esterases, Est_p1 played a notable role in specificity for substrate C4 (kcat/Km value 11,500 S-1m M-1) and showed no inhibited by phenylmethylsulfonyl fluoride, suggested that the substrate binding pocket was suitable for substrate C4 and the serine active-site residue was buried at the bottom of substrate binding pocket which sheltered by a lid structure.
conclusionsEsterase, which specificity towards short chain fatty acids, especially butanoic acid, is commercially available as potent flavoring tools. According the outstanding activity and specificity for substrate C4, Est_p1 has potential application in flavor industries requiring hydrolysis of short chain esters.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.