ArticleBiophysical journal2011
Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- The mechanopathology of the tumor microenvironment: detection techniques, molecular mechanisms and therapeutic opportunities.Frontiers in cell and developmental biology · 2025Review
- Article
- Quantification of ruffle area and dynamics in live or fixed lung adenocarcinoma cells.STAR protocols · 2022Article
- Variable fluid flow regimes alter human brain microvascular endothelial cell-cell junctions and cytoskeletal structure.Cytoskeleton (Hoboken, N.J.) · 2021Article
- Tumor-on-a-chip for integrating a 3D tumor microenvironment: chemical and mechanical factors.Lab on a chip · 2020Review
- A NUMB-EFA6B-ARF6 recycling route controls apically restricted cell protrusions and mesenchymal motility.The Journal of cell biology · 2018Article
- A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.Nature communications · 2018Article
- How can we predict cellular mechanosensation?: Comment on "Cellular mechanosensing of the biophysical microenvironment: A review of mathematical models of biophysical regulation of cell responses" by Bo Cheng et al.Physics of life reviews · 2017Article
- Cellular mechanosensing of the biophysical microenvironment: A review of mathematical models of biophysical regulation of cell responses.Physics of life reviews · 2017Review
- Regulation of circular dorsal ruffles, macropinocytosis, and cell migration by RhoG and its exchange factor, Trio.Molecular biology of the cell · 2017Article
- Fronts and waves of actin polymerization in a bistability-based mechanism of circular dorsal ruffles.Nature communications · 2017Article
- Tuning cell migration: contractility as an integrator of intracellular signals from multiple cues.F1000Research · 2016Review
- Tissue stiffness regulates serine/arginine-rich protein-mediated splicing of the extra domain B-fibronectin isoform in tumors.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Dynamics of actin waves on patterned substrates: a quantitative analysis of circular dorsal ruffles.PloS one · 2015Article
- Galectin-3- and phospho-caveolin-1-dependent outside-in integrin signaling mediates the EGF motogenic response in mammary cancer cells.Molecular biology of the cell · 2013Article
- Substrate Stiffness Regulates PDGF-Induced Circular Dorsal Ruffle Formation Through MLCK.Cellular and molecular bioengineering · 2013Article
- Traveling waves in actin dynamics and cell motility.Current opinion in cell biology · 2013Review
- A kinetic model for RNA-interference of focal adhesions.BMC systems biology · 2013Article
- Mechanistic insights into the regulation of circular dorsal ruffle formation.Journal of biochemistry · 2013Review
- Functions and regulation of circular dorsal ruffles.Molecular and cellular biology · 2012Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circular dorsal ruffles (CDRs) are transient actin-rich ringlike structures that form on the dorsal surface of growth-factor stimulated cells. However, the dynamics and mechanism of formation of CDRs are still unknown. It has been observed that CDR formation leads to stress fibers disappearing near the CDRs. Because stress fiber formation can be modified by substrate stiffness, we examined the effect of substrate stiffness on CDR formation by seeding NIH 3T3 fibroblasts on glass and polydimethylsiloxane substrates of varying stiffnesses from 20 kPa to 1800 kPa. We found that increasing substrate stiffness increased the lifetime of the CDRs. We developed a mathematical model of the signaling pathways involved in CDR formation to provide insight into this lifetime and size dependence that is linked to substrate stiffness via Rac-Rho antagonism. From the model, increasing stiffness raised mDia1-nucleated stress fiber formation due to Rho activation. The increased stress fibers present increased replenishment of the G-actin pool, therefore prolonging Arp2/3-nucleated CDR formation due to Rac activation. Negative feedback by WAVE-related RacGAP on Rac explained how CDR actin propagates as an excitable wave, much like wave propagation in other excitable medium, e.g., nerve signal transmission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.