ArticlePloS one2011
Normal mouse intestinal epithelial cells as a model for the in vitro invasion of Trichinella spiralis infective larvae.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 73 citations in OpenAlex.
- CB2R agonism protects intestinal epithelium through β-catenin/HoxA10 loop in radiation injury.Journal of translational medicine · 2026Article
- Effects of Trichinella spiralis and its serine protease inhibitors on intestinal mucosal barrier function.Veterinary research · 2025Article
- Dual activation of GCGR/GLP1R signaling ameliorates intestinal fibrosisActa pharmaceutica Sinica. B · 2025Article
- An Adaptable Protocol to Generate a Murine Enteroid-Macrophage Co-Culture System.International journal of molecular sciences · 2024Article
- In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host's intestinal epithelial cells.PLoS neglected tropical diseases · 2023Article
- Epidemiology Study ofArchives of Razi Institute · 2023Article
- Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame.Cellular and molecular life sciences : CMLS · 2022Article
- Horizontal gene transfer provides insights into the deep evolutionary history and biology ofFood and waterborne parasitology · 2022Article
- Characterization of a novel cysteine protease in Trichinella spiralis and its role in larval intrusion, development and fecundity.Veterinary research · 2021Article
- Biological properties and roles of a Trichinella spiralis inorganic pyrophosphatase in molting and developmental process of intestinal larval stages.Veterinary research · 2021Article
- Characterization of a Novel Glutamine Synthetase FromFrontiers in cell and developmental biology · 2021Article
- Disruption of Epithelial Barrier of Caco-2 Cell Monolayers by Excretory Secretory Products ofFrontiers in microbiology · 2021Article
- RNAi-mediated silencing of Trichinella spiralis serpin-type serine protease inhibitors results in a reduction in larval infectivity.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis serine proteinase.Veterinary research · 2020Article
- Vaccination of Mice with a Novel Trypsin fromVaccines · 2020Article
- Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity.Veterinary research · 2020Article
- The cysteine protease ATG4B of Trichinella spiralis promotes larval invasion into the intestine of the host.Veterinary research · 2020Article
- Protective immunity in mice vaccinated with a novel elastase-1 significantly decreases Trichinella spiralis fecundity and infection.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis.Parasites & vectors · 2020Article
- Organoids - New Models for Host-Helminth Interactions.Trends in parasitology · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been known for many years that Trichinella spiralis initiates infection by penetrating the columnar epithelium of the small intestine; however, the mechanisms used by the parasite in the establishment of its intramulticellular niche in the intestine are unknown. Although the previous observations indicated that invasion also occurs in vitro when the infective larvae are inoculated onto cultures of intestinal epithelial cells (e.g., human colonic carcinoma cell line Caco-2, HCT-8), a normal readily manipulated in vitro model has not been established because of difficulties in the culture of primary intestinal epithelial cells (IECs). In this study, we described a normal intestinal epithelial model in which T. spiralis infective larvae were shown to invade the monolayers of normal mouse IECs in vitro. The IECs derived from intestinal crypts of fetal mouse small intestine had the ability to proliferate continuously and express specific cytokeratins as well as intestinal functional cell markers. Furthermore, they were susceptible to invasion by T. spiralis. When inoculated onto the IEC monolayer, infective larvae penetrated cells and migrated through them, leaving trails of damaged cells heavily loaded with T. spiralis larval excretory-secretory (ES) antigens which were recognized by rabbit immune sera on immunofluorescence test. The normal intestinal epithelial model of invasion mimicking the natural environment in vivo will help us to further investigate the process as well as the mechanisms by which T. spiralis establishes its intestinal niche.
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