Evidence map›Paper›PMID 22065916›Full record

ArticleMolecular vision2011

Protein changes in the retina following experimental retinal detachment in rabbits.

Nakul Mandal, Geoffrey P Lewis, Steven K Fisher, Steffen Heegaard, Jan U Prause, Morten la Cour, Henrik Vorum, Bent Honoré

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Nakul MandalEye Pathology Section, Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark. nm@doctors.org.uk
Geoffrey P Lewis
Steven K Fisher
Steffen Heegaard
Jan U Prause
Morten la Cour
Henrik Vorum
Bent Honoré
University of Copenhagen · DK

Funding

THE DEVELOPING AND ADULT VISUAL SYSTEMR01EY000888 · NEI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI FISHER, STEVEN K · 1985 to 2008
$3.7M
THE DEVELOPING AND ADULT VISUAL SYSTEMR37EY000888 · NEI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI FISHER, STEVEN K · 1990 to 1999
–
NEI NIH HHS EY-000888NEI NIH HHS R01 EY000888NEI NIH HHS R37 EY000888
6 · The paper itself

Abstract

purposeRetinal detachment leads to the widespread cellular remodeling of the retina. The purpose of this study was to identify protein changes that accompany these cellular alterations by comparing the proteomic profiles of sham and experimentally detached rabbit retina. Elucidation of the proteins most dramatically affected by retinal detachment would add further understanding to the pathophysiology of this condition, and potentially identify therapeutic targets useful in preventing the deleterious effects of detachment, including photoreceptor cell death and the activation of non-neuronal microglial and Müller cells.

methodsRetinal detachments were induced in the right eyes of six New Zealand Red pigmented rabbits. Sham surgery was performed in the right eyes of six other rabbits that were used as controls. At seven days, the eyes were enucleated and the retinal tissue was harvested. The individual retinal samples were subjected to high resolution two-dimensional polyacrylamide gel electrophoresis. Differentially expressed protein spots were processed for identification by liquid chromatography-tandem mass spectrometry. Further investigation was undertaken with western blotting, and immunocytochemical studies on a further set of four sham and four detached retinas.

resultsEighteen protein spots were found to be at least twofold differentially expressed between the sham and detached retinas. These protein spots were identified as: vimentin; tubulin β-2C; fragments of α-enolase; fructose-bisphosphate aldolase A; ATP synthase subunit β; mitochondrial creatine kinase; N-terminal fragments of albumin; prohibitin; and transducin-β(1).

conclusionsThe differentially expressed proteins determined in this study may play an important role in the cellular responses of the retina after its detachment, subsequent ability to recover following surgical reattachment, as well as in serious complications such as subretinal fibrosis and proliferative vitreoretinopathy.

Indexed as

ProteomicsAnimalsBlotting, WesternChromatography, LiquidDisease Models, AnimalElectrophoresis, Gel, Two-DimensionalEye ProteinsGene Expression ProfilingRabbitsRetinaRetinal DetachmentTandem Mass SpectrometryEye Proteins

Identifiers

PMID22065916
PMCPMC3209431
OpenAlexW1825304006

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.