ArticleProceedings of the National Academy of Sciences of the United States of America2011
RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 149 citations in OpenAlex.
- Antisense Oligonucleotide-MediatedAnimals : an open access journal from MDPI · 2026Article
- Epigenetics in development, disease, and therapy: from mechanisms to clinical interventions.Science China. Life sciences · 2026Review
- 5-Hydroxymethylcytosine Dynamics Reveals Coordinated Reprogramming of Parental Genomes and X Chromosome Dosage Balance in Mouse SCNT Embryos.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- LncRNA Xist acts as a miR-486-5p sponge to modulate myoblast proliferation by recruiting Stk4.Journal of muscle research and cell motility · 2025Article
- mTORC1-dependent suppression of autophagic activity in somatic cell nuclear transfer mouse embryos.Reproduction (Cambridge, England) · 2025Article
- Exploring the associations between prenatal PCB exposures and gene expression: Observations from a study of newborn Slovak infants.Ecotoxicology and environmental safety · 2025Article
- Generation of live mice from haploid ESCs with germline-DMR deletions or switch.Cell discovery · 2025Article
- Creation of true interspecies hybrids: Rescue of hybrid class with hybrid cytoplasm affecting growth and metabolism.Science advances · 2024Article
- Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos.National science review · 2023Article
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years.The Journal of reproduction and development · 2023Article
- Early Cell Specification in Mammalian Fertilized and Somatic Cell Nuclear Transfer Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Cloning by SCNT: Integrating Technical and Biology-Driven Advances.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Epigenetic Reprogramming and Somatic Cell Nuclear Transfer.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Knockdown of YY1 InhibitsInternational journal of molecular sciences · 2022Article
- Aberrant nucleosome organization in mouse SCNT embryos revealed by ULI-MNase-seq.Stem cell reports · 2022Article
- Noncanonical imprinting sustains embryonic development and restrains placental overgrowth.Genes & development · 2022Article
- Strategies to Improve the Efficiency of Somatic Cell Nuclear Transfer.International journal of molecular sciences · 2022Review
- Epigenetic manipulation to improve mouse SCNT embryonic development.Frontiers in genetics · 2022Review
- Enhancement of Chromatin and Epigenetic Reprogramming in Porcine SCNT Embryos-Progresses and Perspectives.Frontiers in cell and developmental biology · 2022Article
23 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient. Most SCNT-generated embryos die after implantation because of unidentified, complex epigenetic errors in the process of postimplantation embryonic development. Here we identify the most upstream level of dysfunction leading to impaired development of clones by using RNAi against Xist, a gene responsible for X chromosome inactivation (XCI). A prior injection of Xist-specific siRNA into reconstructed oocytes efficiently corrected SCNT-specific aberrant Xist expression at the morula stage, but failed to do so thereafter at the blastocyst stage. However, we found that shortly after implantation, this aberrant XCI status in cloned embryos had been corrected autonomously in both embryonic and extraembryonic tissues, probably through a newly established XCI control for postimplantation embryos. Embryo transfer experiments revealed that siRNA-treated embryos showed 10 times higher survival than controls as early as embryonic day 5.5 and this high survival persisted until term, resulting in a remarkable improvement in cloning efficiency (12% vs. 1% in controls). Importantly, unlike control clones, these Xist-siRNA clones at birth showed only a limited dysregulation of their gene expression, indicating that correction of Xist expression in preimplantation embryos had a long-term effect on their postnatal normality. Thus, contrary to the general assumption, our results suggest that the fate of cloned embryos is determined almost exclusively before implantation by their XCI status. Furthermore, our strategy provides a promising breakthrough for mammalian SCNT cloning, because RNAi treatment of oocytes is readily applicable to most mammal species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.