ArticleThe Journal of biological chemistry2012
Signal-dependent hydrolysis of phosphatidylinositol 4,5-bisphosphate without activation of phospholipase C: implications on gating of Drosophila TRPL (transient receptor potential-like) channel.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Diacylglycerol Activates theInternational journal of molecular sciences · 2023Article
- Endocannabinoids produced in photoreceptor cells in response to light activateScience signaling · 2022Article
- The Role of Membrane Lipids in Light-Activation ofBiomolecules · 2022Review
- Modulation of Transient Receptor Potential C Channel Activity by Cholesterol.Frontiers in pharmacology · 2019Review
- Speed and sensitivity of phototransduction in Drosophila depend on degree of saturation of membrane phospholipids.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Dynamics of receptor-operated Ca(2+) currents through TRPC channels controlled via the PI(4,5)P2-PLC signaling pathway.Frontiers in pharmacology · 2015Article
- Feeling the hidden mechanical forces in lipid bilayer is an original sense.Proceedings of the National Academy of Sciences of the United States of America · 2014Review
- Diacylglycerol activates the light-dependent channel TRP in the photosensitive microvilli of Drosophila melanogaster photoreceptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Regulation of transient receptor potential channels by the phospholipase C pathway.Advances in biological regulation · 2013Review
- Common mechanisms regulating dark noise and quantum bump amplification in Drosophila photoreceptors.Journal of neurophysiology · 2013Article
- Drosophila TRP channels and animal behavior.Life sciences · 2013Review
- TRPV1 channels are intrinsically heat sensitive and negatively regulated by phosphoinositide lipids.Neuron · 2013Article
- Article
- Drosophila visual transduction.Trends in neurosciences · 2012Review
- TRP, TRPL and cacophony channels mediate Ca2+ influx and exocytosis in photoreceptors axons in Drosophila.PloS one · 2012Article
- The activity of the TRP-like channel depends on its expression system.Channels (Austin, Tex.)Article
- Phototransduction ineNeuroArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In Drosophila, a phospholipase C (PLC)-mediated signaling cascade, couples photo-excitation of rhodopsin to the opening of the transient receptor potential (TRP) and TRP-like (TRPL) channels. A lipid product of PLC, diacylglycerol (DAG), and its metabolites, polyunsaturated fatty acids (PUFAs) may function as second messengers of channel activation. However, how can one separate between the increase in putative second messengers, change in pH, and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) depletion when exploring the TRPL gating mechanism? To answer this question we co-expressed the TRPL channels together with the muscarinic (M1) receptor, enabling the openings of TRPL channels via G-protein activation of PLC. To dissect PLC activation of TRPL into its molecular components, we used a powerful method that reduced plasma membrane-associated PI(4,5)P(2) in HEK cells within seconds without activating PLC. Upon the addition of a dimerizing drug, PI(4,5)P(2) was selectively hydrolyzed in the cell membrane without producing DAG, inositol trisphosphate, or calcium signals. We show that PI(4,5)P(2) is not an inhibitor of TRPL channel activation. PI(4,5)P(2) hydrolysis combined with either acidification or application of DAG analogs failed to activate the channels, whereas PUFA did activate the channels. Moreover, a reduction in PI(4,5)P(2) levels or inhibition of DAG lipase during PLC activity suppressed the PLC-activated TRPL current. This suggests that PI(4,5)P(2) is a crucial substrate for PLC-mediated activation of the channels, whereas PUFA may function as the channel activator. Together, this study defines a narrow range of possible mechanisms for TRPL gating.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.