Trial reportDiabetologia2012
Branched-chain amino acid levels are associated with improvement in insulin resistance with weight loss.
Trial report in Diabetologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 210 papers, 5 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
210 citing papers in PubMed, 5 syntheses or guidelines pooled it, 347 citations in OpenAlex.
- Metabolomic profiling identifies complex lipid species and amino acid analogues associated with response to weight loss interventions.PloS one · 2021Pooled it
- Profiling of Endogenous and Gut Microbial Metabolites to Indicate Metabotype-Specific Dietary Responses: A Systematic Review.Advances in nutrition (Bethesda, Md.) · 2020Pooled it
- Cumulative consumption of branched-chain amino acids and incidence of type 2 diabetes.International journal of epidemiology · 2016Pooled it
- Metabolomics in Prediabetes and Diabetes: A Systematic Review and Meta-analysis.Diabetes care · 2016Pooled it
- The Relationship between Branched-Chain Amino Acid Related Metabolomic Signature and Insulin Resistance: A Systematic Review.Journal of diabetes research · 2016Pooled it
- Impact of comprehensive lifestyle interventions on plasma branched-chain amino acid concentrations: a randomized trial.The American journal of clinical nutrition · 2025Trial
- Amino acid composition of plant protein-enriched wheat biscuits differentially affects postprandial amino acid responses of overweight/obese compared to normalweight subjects.European journal of nutrition · 2025Trial
- The impact of leucine supplementation on body composition and glucose tolerance following energy restriction: an 8-week RCT in adults at risk of the metabolic syndrome.European journal of clinical nutrition · 2024Trial
- Diet-induced Weight Loss and Phenotypic Flexibility Among Healthy Overweight Adults: A Randomized Trial.The American journal of clinical nutrition · 2023Trial
- Plasma metabolites associated with functional and clinical outcomes in heart failure with reduced ejection fraction with and without type 2 diabetes.Scientific reports · 2022Trial
- Metabolites and diabetes remission after weight loss.Nutrition & diabetes · 2021Trial
- A Novel Dietary Intervention Reduces Circulatory Branched-Chain Amino Acids by 50%: A Pilot Study of Relevance for Obesity and Diabetes.Nutrients · 2020Trial
- Decreased adipose tissue oxygenation associates with insulin resistance in individuals with obesity.The Journal of clinical investigation · 2020Trial
- Associations between Plasma Branched Chain Amino Acids and Health Biomarkers in Response to Resistance Exercise Training Across Age.Nutrients · 2020Trial
- Pre-meal protein intake alters postprandial plasma metabolome in subjects with metabolic syndrome.European journal of nutrition · 2020Trial
- Preliminary evidence of effects of potassium chloride on a metabolomic path to diabetes and cardiovascular disease.Metabolomics : Official journal of the Metabolomic Society · 2020Trial
- Trial
- Circulating amino acids and the risk of macrovascular, microvascular and mortality outcomes in individuals with type 2 diabetes: results from the ADVANCE trial.Diabetologia · 2018Trial
- A novel insulin resistance index to monitor changes in insulin sensitivity and glucose tolerance: the ACT NOW study.The Journal of clinical endocrinology and metabolism · 2015Trial
- Diets high in protein or saturated fat do not affect insulin sensitivity or plasma concentrations of lipids and lipoproteins in overweight and obese adults.The Journal of nutrition · 2014Trial
150 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors at 10 institutions in 1 country.
Funding
Abstract
aims/hypothesisInsulin resistance (IR) improves with weight loss, but this response is heterogeneous. We hypothesised that metabolomic profiling would identify biomarkers predicting changes in IR with weight loss.
methodsTargeted mass spectrometry-based profiling of 60 metabolites, plus biochemical assays of NEFA, β-hydroxybutyrate, ketones, insulin and glucose were performed in baseline and 6 month plasma samples from 500 participants who had lost ≥4 kg during Phase I of the Weight Loss Maintenance (WLM) trial. Homeostatic model assessment of insulin resistance (HOMA-IR) and change in HOMA-IR with weight loss (∆HOMA-IR) were calculated. Principal components analysis (PCA) and mixed models adjusted for race, sex, baseline weight, and amount of weight loss were used; findings were validated in an independent cohort of patients (n = 22).
resultsMean weight loss was 8.67 ± 4.28 kg; mean ∆HOMA-IR was -0.80 ± 1.73, range -28.9 to 4.82). Baseline PCA-derived factor 3 (branched chain amino acids [BCAAs] and associated catabolites) correlated with baseline HOMA-IR (r = 0.50, p < 0.0001) and independently associated with ∆HOMA-IR (p < 0.0001). ∆HOMA-IR increased in a linear fashion with increasing baseline factor 3 quartiles. Amount of weight loss was only modestly correlated with ∆HOMA-IR (r = 0.24). These findings were validated in the independent cohort, with a factor composed of BCAAs and related metabolites predicting ∆HOMA-IR (p = 0.007). CONCLUSIONS/
interpretationA cluster of metabolites comprising BCAAs and related analytes predicts improvement in HOMA-IR independent of the amount of weight lost. These results may help identify individuals most likely to benefit from moderate weight loss and elucidate novel mechanisms of IR in obesity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.