ArticleInvestigative ophthalmology & visual science2011
Substratum compliance regulates human trabecular meshwork cell behaviors and response to latrunculin B.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Investigating the coupled effects of stiffness and stretch on the trabecular meshwork cells using a hydrogel-integrated microfluidic system.Biomaterials science · 2026Article
- Bioengineered 3D Human Trabecular Meshwork Models for Outflow Physiology and Glaucoma Research.Bioengineering (Basel, Switzerland) · 2026Review
- Extracellular vesicles as emerging players in glaucoma: Mechanisms, biomarkers, and therapeutic targets.Vision research · 2025Review
- Photoinduced Gelatin-Methacrylate Scaffolds to Examine the Impact of Extracellular Environment on Trabecular Meshwork Cells.Industrial & engineering chemistry research · 2021Article
- Normal and glaucomatous outflow regulation.Progress in retinal and eye research · 2021Review
- Dexamethasone and Glucocorticoid-Induced Matrix Temporally Modulate Key Integrins, Caveolins, Contractility, and Stiffness in Human Trabecular Meshwork Cells.Investigative ophthalmology & visual science · 2020Article
- Generating cell-derived matrices from human trabecular meshwork cell cultures for mechanistic studies.Methods in cell biology · 2020Article
- YAP and TAZ are distinct effectors of corneal myofibroblast transformation.Experimental eye research · 2019Article
- Latrunculin B and substratum stiffness regulate corneal fibroblast to myofibroblast transformation.Experimental eye research · 2018Article
- YAP and TAZ mediate steroid-induced alterations in the trabecular meshwork cytoskeleton in human trabecular meshwork cells.International journal of molecular medicine · 2018Article
- The many faces of the trabecular meshwork cell.Experimental eye research · 2017Review
- OCT Study of Mechanical Properties Associated with Trabecular Meshwork and Collector Channel Motion in Human Eyes.PloS one · 2016Article
- Biomechanical assessment in models of glaucomatous optic neuropathy.Experimental eye research · 2015Review
- Dexamethasone Stiffens Trabecular Meshwork, Trabecular Meshwork Cells, and Matrix.Investigative ophthalmology & visual science · 2015Article
- The intrinsic stiffness of human trabecular meshwork cells increases with senescence.Oncotarget · 2015Article
- Altered mechanobiology of Schlemm's canal endothelial cells in glaucoma.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Elastic modulus and collagen organization of the rabbit cornea: epithelium to endothelium.Acta biomaterialia · 2014Article
- What do mechanotransduction, Hippo, Wnt, and TGFβ have in common? YAP and TAZ as key orchestrating molecules in ocular health and disease.Experimental eye research · 2013Review
- Substratum compliance modulates corneal fibroblast to myofibroblast transformation.Investigative ophthalmology & visual science · 2013Article
- Substratum stiffness and latrunculin B modulate the gene expression of the mechanotransducers YAP and TAZ in human trabecular meshwork cells.Experimental eye research · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine the impact of substratum compliance and latrunculin-B (Lat-B), both alone and together, on fundamental human trabecular meshwork (HTM) cell behavior. Lat-B is a reversible actin cytoskeleton disruptor that decreases resistance to aqueous humor outflow and decreases intraocular pressure.
methodsHTM cells were cultured on polyacrylamide hydrogels possessing values for compliance that mimic those reported for normal and glaucomatous HTM, or tissue culture plastic (TCP). Cells were treated with 0.2 μM or 2.0 μM Lat-B in dimethyl sulfoxide (DMSO) or DMSO alone. The impact of substratum compliance and/or Lat-B treatment on cell attachment, proliferation, surface area, aspect ratio, and migration were investigated.
resultsHTM cells had profoundly decreased attachment and proliferation rates when cultured on hydrogels possessing compliance values that mimic those found for healthy HTM. The effect of Lat-B treatment on HTM cell surface area was less for cells cultured on more compliant hydrogels compared with TCP. HTM cell migration was increased on stiffer hydrogels that mimic the compliance of glaucomatous HTM and on TCP in comparison with more compliant hydrogels. Lat-B treatment decreased cellular migration on all surfaces for at least 7 hours after treatment.
conclusionsSubstratum compliance profoundly influenced HTM cell behaviors and modulated the response of HTM cells to Lat-B. The inclusion of substratum compliance that reflects healthy or glaucomatous HTM results in cell behaviors and responses to therapeutic agents in vitro that may more accurately reflect in vivo conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.