ArticleHuman molecular genetics2012
Derivation of new human embryonic stem cell lines reveals rapid epigenetic progression in vitro that can be prevented by chemical modification of chromatin.
Article in Human molecular genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 60 citations in OpenAlex.
- Human primordial germ cell heterogeneity in vitro is associated with distinctive biological states.Cellular & molecular biology letters · 2026Article
- TFAP2A+ embryonic progenitor cells undergo fate diversification to give rise to human amnion, germline, and mesoderm.bioRxiv : the preprint server for biology · 2025Article
- ZIC2 and ZIC3 promote SWI/SNF recruitment to safeguard progression towards human primed pluripotency.Nature communications · 2024Article
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- Article
- Early chromosome condensation by XIST builds A-repeat RNA density that facilitates gene silencing.Cell reports · 2023Article
- Article
- Preventing erosion of X-chromosome inactivation in human embryonic stem cells.Nature communications · 2022Article
- Divergent roles for KLF4 and TFCP2L1 in naive ground state pluripotency and human primordial germ cell development.Stem cell research · 2021Article
- Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research.Stem cell research · 2021Article
- Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research.Stem cell research · 2021Article
- Female human primordial germ cells display X-chromosome dosage compensation despite the absence of X-inactivation.Nature cell biology · 2020Article
- Naive Human Embryonic Stem Cells Can Give Rise to Cells with a Trophoblast-like Transcriptome and Methylome.Stem cell reports · 2020Article
- Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors.Cell reports · 2019Article
- Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells.Cell stem cell · 2019Article
- The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation.Cell reports · 2018Article
- TFAP2C regulates transcription in human naive pluripotency by opening enhancers.Nature cell biology · 2018Article
- PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation.Stem cell research · 2018Article
- TRIM28-Regulated Transposon Repression Is Required for Human Germline Competency and Not Primed or Naive Human Pluripotency.Stem cell reports · 2018Article
- Human X chromosome inactivation and reactivation: implications for cell reprogramming and disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Human embryonic stem cells (hESCs) are pluripotent cell types derived from the inner cell mass of human blastocysts. Recent data indicate that the majority of established female XX hESC lines have undergone X chromosome inactivation (XCI) prior to differentiation, and XCI of hESCs can be either XIST-dependent (class II) or XIST-independent (class III). XCI of female hESCs precludes the use of XX hESCs as a cell-based model for examining mechanisms of XCI, and will be a challenge for studying X-linked diseases unless strategies are developed to reactivate the inactive X. In order to recover nuclei with two active X chromosomes (class I), we developed a reprogramming strategy by supplementing hESC media with the small molecules sodium butyrate and 3-deazaneplanocin A (DZNep). Our data demonstrate that successful reprogramming can occur from the XIST-dependent class II nuclear state but not class III nuclear state. To determine whether these small molecules prevent XCI, we derived six new hESC lines under normoxic conditions (UCLA1-UCLA6). We show that class I nuclei are present within the first 20 passages of hESC derivation prior to cryopreservation, and that supplementation with either sodium butyrate or DZNep preserve class I nuclei in the self-renewing state. Together, our data demonstrate that self-renewal and survival of class I nuclei are compatible with normoxic hESC derivation, and that chemical supplementation after derivation provides a strategy to prevent epigenetic progression and retain nuclei with two active X chromosomes in the self-renewing state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.