ArticleApplied and environmental microbiology2012
Rapid identification of Pseudallescheria and Scedosporium strains by using rolling circle amplification.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- An updated list of eumycetoma causative agents and their differences in grain formation and treatment response.Clinical microbiology reviews · 2024Review
- Mycetoma: Development of Diagnosis and Treatment.Journal of fungi (Basel, Switzerland) · 2022Review
- Development and Evaluation of Isothermal Amplification Methods for Rapid Detection of LethalFrontiers in microbiology · 2019Article
- Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes.Mycopathologia · 2018Article
- Challenges in Laboratory Detection of Fungal Pathogens in the Airways of Cystic Fibrosis Patients.Mycopathologia · 2018Article
- Azole-Resistance inFrontiers in microbiology · 2018Article
- In Vivo Synergy of Amphotericin B plus Posaconazole in Murine Aspergillosis.Antimicrobial agents and chemotherapy · 2016Article
- Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification.Frontiers in microbiology · 2015Article
- Rapid identification of black grain eumycetoma causative agents using rolling circle amplification.PLoS neglected tropical diseases · 2014Article
- Merits and pitfalls of currently used diagnostic tools in mycetoma.PLoS neglected tropical diseases · 2014Review
- In vitro antifungal susceptibility of Candida glabrata to caspofungin and the presence of FKS mutations correlate with treatment response in an immunocompromised murine model of invasive infection.Antimicrobial agents and chemotherapy · 2014Article
- Externa otitis caused by the Graphium stage of Pseudallescheria apiosperma.Medical mycology case reports · 2013Article
- Identification and typing of isolates of Cyphellophora and relatives by use of amplified fragment length polymorphism and rolling circle amplification.Journal of clinical microbiology · 2013Article
- Identification of Pseudallescheria and Scedosporium species by three molecular methods.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Pseudallescheria boydii complex, comprising environmental pathogens with Scedosporium anamorphs, has recently been subdivided into five main species: Scedosporium dehoogii, S. aurantiacum, Pseudallescheria minutispora, P. apiosperma, and P. boydii, while the validity of some other taxa is being debated. Several Pseudallescheria and Scedosporium species are indicator organisms of pollution in soil and water. Scedosporium dehoogii in particular is enriched in soils contaminated by aliphatic hydrocarbons. In addition, the fungi may cause life-threatening infections involving the central nervous system in severely impaired patients. For screening purposes, rapid and economic tools for species recognition are needed. Our aim is to establish rolling circle amplification (RCA) as a screening tool for species-specific identification of Pseudallescheria and Scedosporium. With this aim, a set of padlock probes was designed on the basis of the internal transcribed spacer (ITS) region, differing by up to 13 fixed mutations. Padlock probes were unique as judged from sequence comparison by BLAST search in GenBank and in dedicated research databases at CBS (Centraalbureau voor Schimmelcultures Fungal Biodiversity Centre). RCA was applied as an in vitro tool, tested with pure DNA amplified from cultures. The species-specific padlock probes designed in this study yielded 100% specificity. The method presented here was found to be an attractive alternative to identification by restriction fragment length polymorphism (RFLP) or sequencing. The rapidity (<1 day), specificity, and low costs make RCA a promising screening tool for environmentally and clinically relevant fungi.
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