ArticleMethods in molecular biology (Clifton, N.J.)2012
Carbohydrate antigen microarrays.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Probing the Broad-Spectrum Virus-Neutralizing Epitopes Using the High-Density Oligomannose-Conjugates and Carbohydrate Microarrays.Bioconjugate chemistry · 2025Article
- Serum antibody screening using glycan arrays.Chemical Society reviews · 2024Review
- Coronaviruses' sugar shields as vaccine candidates.Current trends in immunology · 2020Article
- Factors contributing to variability of glycan microarray binding profiles.Faraday discussions · 2019Article
- Factors Affecting Anti-Glycan IgG and IgM Repertoires in Human Serum.Scientific reports · 2016Article
- Targeting N-glycan cryptic sugar moieties for broad-spectrum virus neutralization: progress in identifying conserved molecular targets in viruses of distinct phylogenetic origins.Molecules (Basel, Switzerland) · 2015Article
- Uncovering cryptic glycan markers in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE).Drug development research · 2014Article
- Chemistry of natural glycan microarrays.Current opinion in chemical biology · 2014Review
- Article
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Abstract
This chapter describes one of my laboratory's working protocols for carbohydrate-based microarrays. Using a standard microarray spotter, we print carbohydrate antigens directly on the nitrocellulose-coated bioarray substrates. Because these substrates support noncovalent immobilization of many spotted antigens, in general no chemical modification of the antigen is needed for microarray production. Thus, this bioarray platform is technically simple and applicable for high-throughput construction of carbohydrate antigen microarrays. A number of nitrocellulose-coated glass slides with different technical characteristics are commercially available. Given the structural diversity of carbohydrate antigens, examining each antigen preparation to determine the efficacy of its immobilization in a given type of substrate and the surface display of the desired glycoepitopes in a microarray assay is essential.
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