Evidence map›Paper›PMID 22057524›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2012

General procedure for the synthesis of neoglycoproteins and immobilization on epoxide-modified glass slides.

Yalong Zhang, Jeffrey C Gildersleeve

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Serum antibody screening using glycan arrays.Chemical Society reviews · 2024
    Review
  4. Article
  5. Article
  6. Glycan Array Technology.Advances in biochemical engineering/biotechnology · 2021
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Antibody repertoire profiling with mimotope arrays.Human vaccines & immunotherapeutics · 2017
    Article
  14. Glycomaterials for probing host-pathogen interactions and the immune response.Experimental biology and medicine (Maywood, N.J.) · 2016
    Review
  15. The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens.Methods in molecular biology (Clifton, N.J.) · 2015
    Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yalong ZhangChemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.
Jeffrey C Gildersleeve

Funding

Development of a Carbohydrate MicroarrayZIABC010675 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI GILDERSLEEVE, JEFFREY · 2009 to 2025
$6.1M
Development of a Carbohydrate MicroarrayZ01BC010675 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI GILDERSLEEVE, JEFF · 2005 to 2008
$438k
Intramural NIH HHS Z01 BC010675
6 · The paper itself

Abstract

Neoglycoproteins, such as BSA-glycosides, contain carbohydrates covalently attached to a protein carrier via nonnaturally occurring linkages. These conjugates have been used for decades to study carbohydrate-protein interactions and are frequently used as immunogens to raise antibodies to carbohydrate antigens. In fact, neoglycoproteins have been used extensively as vaccine antigens and several have obtained FDA approval. More recently, neoglycoproteins have been used in the construction of glycan arrays to produce "neoglycoprotein microarrays." In this chapter, two methods for preparing neoglycoproteins are described along with methods to immobilize these conjugates on epoxide-coated glass microscope slides to produce arrays.

Indexed as

Epoxy CompoundsGlassGlycoproteinsProtein Array AnalysisEpoxy CompoundsGlycoproteins

Identifiers

PMID22057524
PMCPMC7461606

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.