ArticleClinical rheumatology2012
A novel automated indirect immunofluorescence autoantibody evaluation.
Article in Clinical rheumatology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- Pilot Study of AI-Assisted ANA Immunofluorescence Reading-Comparison with Classical Visual Interpretation.Journal of clinical medicine · 2025Article
- Charting the Next Road Map for CSF Biomarkers in Alzheimer's Disease and Related Dementias.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2023Review
- Analytical and clinical performance of different platforms simultaneously detecting 15 antinuclear antibodies.Journal of clinical laboratory analysis · 2022Article
- Standardization and Quality Assessment Under the Perspective of Automated Computer-Assisted HEp-2 Immunofluorescence Assay Systems.Frontiers in immunology · 2021Review
- A machine learning algorithm for simulating immunohistochemistry: development of SOX10 virtual IHC and evaluation on primarily melanocytic neoplasms.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2020Article
- Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing.Frontiers in immunology · 2018Article
- Recent Approaches To Optimize Laboratory Assessment of Antinuclear Antibodies.Clinical and vaccine immunology : CVI · 2017Review
- Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology.Clinical reviews in allergy & immunology · 2017Review
- The burden of the variability introduced by the HEp-2 assay kit and the CAD system in ANA indirect immunofluorescence test.Immunologic research · 2017Article
- A paper/polymer hybrid microfluidic microplate for rapid quantitative detection of multiple disease biomarkers.Scientific reports · 2016Article
- The Reliability of a Novel Automated System for ANA Immunofluorescence Analysis in Daily Clinical Practice.International journal of rheumatology · 2016Article
- Review
- A fully automated IIF system for the detection of antinuclear antibodies and antineutrophil cytoplasmic antibodies.Immunologic research · 2015Article
- Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology.Journal of immunology research · 2015Article
- The classification of Crithidia luciliae immunofluorescence test (CLIFT) using a novel automated system.Arthritis research & therapy · 2014Article
- Automated tests of ANA immunofluorescence as throughput autoantibody detection technology: strengths and limitations.BMC medicine · 2014Review
- Novel clinical and diagnostic aspects of antineutrophil cytoplasmic antibodies.Journal of immunology research · 2014Review
- Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA.PloS one · 2014Article
- Current concepts and future directions for the assessment of autoantibodies to cellular antigens referred to as anti-nuclear antibodies.Journal of immunology research · 2014Review
- A comparison of anti-nuclear antibody quantification using automated enzyme immunoassays and immunofluorescence assays.Autoimmune diseases · 2014Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autoantibodies (AAb), especially antinuclear (ANAs) and anticytoplasmatic antibodies (ACyA), are essential diagnosing markers for several autoimmune diseases. The current gold standard method for ANA detection is manual indirect immunofluorescence (IIF) on human epithelial-2 (HEp-2) cells. However, this technique is cost and time consuming, and characterized by considerable intra- and interlaboratory variability. Thus, an automated IIF-HEp-2 reader has been developed recently. In the current study, we compared the performance of the automated AAb IIF-HEp-2 interpretation to conventional detection methods. Autoantibody detection by IIF on HEp-2 cells was performed in a total of 260 sera of patients, including 34 with systemic lupus erythematosus, 111 with dermatomyositis or polymyositis, 74 with systemic sclerosis, 41 with rare AAb patterns, and 137 healthy individuals. Visual interpretation and routine immunoassays were compared with a novel automated IIF-HEp-2 system using Aklides pattern recognition algorithms. Positive AAbs were detected in 95-100% of rheumatic patients by automated interpretation, in 74-100% with manual reading, and in 64-100% by immunodot assay. Receiver operating characteristic curve analysis of fluorescent intensity revealed a high sensitivity and specificity for automated reading of AAb with an agreement ranging from 90% to 95% between manual and automated interpretation (kappa 0.554-0.69) for systemic sclerosis and myositis, respectively. This study demonstrates a good correlation between manual and automated interpretation of AAb including ANA and ACyA in patients with autoimmune diseases. Full automation of HEp-2 cell assay reading may minimize errors in ANA pattern interpretation and thus help in the standardization of ANA assessment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.