ArticleJournal of bacteriology2012
Characterization of DNA sequences required for the CcrAB-mediated integration of staphylococcal cassette chromosome mec, a Staphylococcus aureus genomic island.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 30 citations in OpenAlex.
- Prevalence and antimicrobial susceptibility ofOne health (Amsterdam, Netherlands) · 2026Article
- Staphylococcal Cassette ChromosomeAntibiotics (Basel, Switzerland) · 2026Article
- Genome co-adaptation and the evolution of methicillin resistant Staphylococcus aureus.Genome biology · 2026Article
- Mixed-biofilm natural transformation assay reveals the presence of staphylococci in human environments that can transfer SCCmSphere · 2025Article
- Tandem mobilization of anti-phage defenses alongside SCCmec elements in staphylococci.Nature communications · 2024Article
- Methicillin resistance in Staphylococcus pseudintermedius encoded within novel staphylococcal cassette chromosome mec (SCCmec) variants.The Journal of antimicrobial chemotherapy · 2024Article
- Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms.Nature communications · 2022Article
- Genome-Based Characterization of a Plasmid-Associated Micrococcin P1 Biosynthetic Gene Cluster and Virulence Factors in Mammaliicoccus sciuri IMDO-S72.Applied and environmental microbiology · 2022Article
- Rapid methicillin resistance diversification in Staphylococcus epidermidis colonizing human neonates.Nature communications · 2021Article
- Misidentification of meticillin-resistantEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2021Article
- Complete Genome Sequences of Diverse Uropathogenic Staphylococcus saprophyticus Isolates from a College Health Center.Microbiology resource announcements · 2020Article
- A mecC allotype, mecC3, in the CoNS Staphylococcus caeli, encoded within a variant SCCmecC.The Journal of antimicrobial chemotherapy · 2019Article
- A highly conserved mecC-encoding SCCmec type XI in a bovine isolate of methicillin-resistant Staphylococcus xylosus.The Journal of antimicrobial chemotherapy · 2018Article
- Article
- Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology.Clinical microbiology reviews · 2018Review
- Factors Contributing to the Evolution ofFrontiers in microbiology · 2018Review
- Transfer of the methicillin resistance genomic island among staphylococci by conjugation.Molecular microbiology · 2016Article
- A Look into the Melting Pot: The mecC-Harboring Region Is a Recombination Hot Spot in Staphylococcus stepanovicii.PloS one · 2016Article
- Modulation of ccrAB Expression and SCCmec Excision by an Inverted Repeat Element and SarS in Methicillin-Resistant Staphylococcus aureus.Antimicrobial agents and chemotherapy · 2015Article
- A Plasmid-Borne System To Assess the Excision and Integration of Staphylococcal Cassette Chromosome mec Mediated by CcrA and CcrB.Journal of bacteriology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The mobile element staphylococcal cassette chromosome mec (SCCmec), which carries mecA, the gene responsible for methicillin resistance in staphylococci, inserts into the chromosome at a specific site, attB, mediated by serine recombinases, CcrAB and CcrC, encoded on the element. This study sought to determine the sequence specificity for CcrB DNA binding in vitro and for CcrAB-mediated SCCmec insertion in vivo. CcrB DNA binding, as assessed in vitro by electrophoretic mobility shift assay (EMSA), revealed that a 14-bp sequence (CGTATCATAAGTAA; the terminal sequence of the orfX gene) was the minimal requirement for binding, containing an invariant sequence (TATCATAA) found in all chromosomal (attB) and SCCmec (attS) integration sites. The sequences flanking the minimal attB and attS binding sites required for insertion in vivo were next determined. A plasmid containing only 37 bp of attS and flanking sequences was required for integration into the attB site at 92% efficiency. In contrast, at least 200 bp of sequence within orfX, 5' to the attB core, and 120 bp of specific sequence 3' to the orfX stop site and attB core were required for the highest insertion frequency. Finally, an attS-containing plasmid was inserted into wild-type Staphylococcus aureus strains without integrated SCCmec (methicillin susceptible) at various frequencies which were determined both by sequences flanking the att site and by the presence of more than one att site on either the chromosome or the integration plasmid. This sequence specificity may play a role in the epidemiology of SCCmec acquisition.
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