ArticleNature methods2011
A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 85 citations in OpenAlex.
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- Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice.iScience · 2023Article
- Rhodopsin-Based Optogenetics: Basics and Applications.Methods in molecular biology (Clifton, N.J.) · 2022Article
- PACmn for improved optogenetic control of intracellular cAMP.BMC biology · 2021Article
- BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons.Nature communications · 2021Article
- Modified Rhodopsins FromFrontiers in molecular biosciences · 2021Article
- Optical stimulation of neural tissue.Healthcare technology letters · 2020Article
- Action potentials in Xenopus oocytes triggered by blue light.The Journal of general physiology · 2020Article
- A Visual Two-Choice Rule-Switch Task for Head-Fixed Mice.Frontiers in behavioral neuroscience · 2019Article
- Potassium channel-based optogenetic silencing.Nature communications · 2018Article
- Diminishing fear: Optogenetic approach toward understanding neural circuits of fear control.Pharmacology, biochemistry, and behavior · 2018Review
- Overexpression of Different Types of Microbial Rhodopsins with a Highly Expressible Bacteriorhodopsin from Haloarcula marismortui as a Single Protein in E. coli.Scientific reports · 2018Article
- Light-Driven Chloride Transport Kinetics of Halorhodopsin.Biophysical journal · 2018Article
- protaTETHER - a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc-GFP fusion protein.FEBS open bio · 2018Article
- Dynamic all-optical drug screening on cardiac voltage-gated ion channels.Scientific reports · 2018Article
- A New Promoter Allows Optogenetic Vision Restoration with Enhanced Sensitivity in Macaque Retina.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
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8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precise co-localization and stoichiometric expression of two different light-gated membrane proteins can vastly improve the physiological usefulness of optogenetics for the modulation of cell excitability with light. Here we present a gene-fusion strategy for the stable 1:1 expression of any two microbial rhodopsins in a single polypeptide chain. By joining the excitatory channelrhodopsin-2 with the inhibitory ion pumps halorhodopsin or bacteriorhodopsin, we demonstrate light-regulated quantitative bi-directional control of the membrane potential in HEK293 cells and neurons in vitro. We also present synergistic rhodopsin combinations of channelrhodopsin-2 with Volvox carteri channelrhodopsin-1 or slow channelrhodopsin-2 mutants, to achieve enhanced spectral or kinetic properties, respectively. Finally, we demonstrate the utility of our fusion strategy to determine ion-turnovers of as yet uncharacterized rhodopsins, exemplified for archaerhodopsin and CatCh, or to correct pump cycles, exemplified for halorhodopsin.
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