ArticleJournal of structural biology2012
Formins filter modified actin subunits during processive elongation.
Article in Journal of structural biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 90 citations in OpenAlex.
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- Localization and function of septins are susceptible to epitope tagging.bioRxiv : the preprint server for biology · 2025Article
- Improved tools for live imaging of F-actin structures in yeast.Molecular biology of the cell · 2024Article
- IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms.PLoS biology · 2024Article
- Overexpression of Lifeact in theFrontiers in cell and developmental biology · 2024Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.Biophysical journal · 2023Article
- Dendritic effects of genetically encoded actin-labeling probes in cultured hippocampal neurons.Molecular biology of the cell · 2023Article
- INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury.Nature communications · 2022Article
- mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells.Journal of cell science · 2022Article
- Rapid assembly of a polar network architecture drives efficient actomyosin contractility.Cell reports · 2022Article
- Counting actin in contractile rings reveals novel contributions of cofilin and type II myosins to fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- Article
- Role of formin INF2 in human diseases.Molecular biology reports · 2022Review
- Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Filament-guided filament assembly provides structural memory of filament alignment during cytokinesis.Developmental cell · 2021Article
- Article
- A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin.The Journal of general physiology · 2021Article
- Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring.The Journal of cell biology · 2021Article
- The role of actin and myosin in antigen extraction by B lymphocytes.Seminars in cell & developmental biology · 2020Review
- Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos.Journal of visualized experiments : JoVE · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Fission yeast cells reject actin subunits tagged with a fluorescent protein from the cytokinetic contractile ring, so cytokinesis fails and the cells die when the native actin gene is replaced by GFP-actin. The lack of a fluorescent actin probe has prevented a detailed study of actin filament dynamics in contractile rings, and left open questions regarding the mechanism of cytokinesis. To incorporate fluorescent actin into the contractile ring to study its dynamics, we introduced the coding sequence for a tetracysteine motif (FLNCCPGCCMEP) at 10 locations in the fission yeast actin gene and expressed the mutant proteins from the native actin locus in diploid cells with wild-type actin on the other chromosome. We labeled these tagged actins inside live cells with the FlAsH reagent. Cells incorporated some of these labeled actins into actin patches at sites of endocytosis, where Arp2/3 complex nucleates all of the actin filaments. However, the cells did not incorporate any of the FlAsH-actins into the contractile ring. Therefore, formin Cdc12p rejects actin subunits with a tag of ~2 kDa, illustrating the stringent structural requirements for this formin to promote the elongation of actin filament barbed ends as it moves processively along the end of a growing filament.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.