ArticleJournal of molecular biology2012
Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Non-canonical cytochrome P450 enzymes expand the diversity of bacterial hemoproteins.Nature chemical biology · 2026Article
- Discovery of noncanonical cytochrome P450 enzymes in nature.Nature chemical biology · 2026Article
- Macrolide antibiotic action, resistance, and tolerance, and approaches to improve their activity against Gram-negative bacteria.The Journal of antibiotics · 2026Review
- Antimicrobial Trioxacarcin 1,2-Dihydroxyanthraquinones from the BacteriumBiomolecules · 2026Article
- Non-Canonical Cytochrome P450 Enzymes in Nature.bioRxiv : the preprint server for biology · 2024Article
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
- Rethinking Biosynthesis of Aclacinomycin A.Molecules (Basel, Switzerland) · 2023Review
- Expression and Purification of Glycosyltransferase DnmS fromMicroorganisms · 2023Article
- Pathway Engineering of Anthracyclines: Blazing Trails in Natural Product Glycodiversification.The Journal of organic chemistry · 2020Article
- Article
- Biosynthetic engineering of the antifungal, anti-MRSA auroramycin.Microbial cell factories · 2020Article
- Enzymatic Synthesis of the C-Glycosidic Moiety of Nogalamycin R.ACS chemical biology · 2018Article
- Cooperative Involvement of Glycosyltransferases in the Transfer of Amino Sugars during the Biosynthesis of the Macrolactam Sipanmycin by Streptomyces sp. Strain CS149.Applied and environmental microbiology · 2018Article
- Crystal structures of eukaryote glycosyltransferases reveal biologically relevant enzyme homooligomers.Cellular and molecular life sciences : CMLS · 2018Review
- Two Cooperative Glycosyltransferases Are Responsible for the Sugar Diversity of Saquayamycins Isolated from Streptomyces sp. KY 40-1.ACS chemical biology · 2017Article
- Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.Natural product reports · 2017Review
- Advances in understanding glycosyltransferases from a structural perspective.Current opinion in structural biology · 2014Review
- Structural studies of the spinosyn forosaminyltransferase, SpnP.Biochemistry · 2014Article
- Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.Applied and environmental microbiology · 2013Article
- Unusual cytochrome p450 enzymes and reactions.The Journal of biological chemistry · 2013Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
In the biosynthesis of the clinically important antibiotic erythromycin D, the glycosyltransferase (GT) EryCIII, in concert with its partner EryCII, attaches a nucleotide-activated sugar to the macrolide scaffold with high specificity. To understand the role of EryCII, we have determined the crystal structure of the EryCIII·EryCII complex at 3.1 Å resolution. The structure reveals a heterotetramer with a distinctive, elongated quaternary organization. The EryCIII subunits form an extensive self-complementary dimer interface at the center of the complex, and the EryCII subunits lie on the periphery. EryCII binds in the vicinity of the putative macrolide binding site of EryCIII but does not make direct interactions with this site. Our biophysical and enzymatic data support a model in which EryCII stabilizes EryCIII and also functions as an allosteric activator of the GT.
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