ArticleThe Journal of allergy and clinical immunology2012
Genetic and epigenetic variations in inducible nitric oxide synthase promoter, particulate pollution, and exhaled nitric oxide levels in children.
Article in The Journal of allergy and clinical immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02469428 (Epigenetic Effect Modifications With Ozone Exposure on Healthy Volunteers), which is not on this map. Cited by 67 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Epigenetic Effect Modifications With Ozone Exposure on Healthy Volunteers
Who cites it
67 citing papers in PubMed, 3 syntheses or guidelines pooled it, 131 citations in OpenAlex.
- The Impact of Air Pollution on Our Epigenome: How Far Is the Evidence? (A Systematic Review).Current environmental health reports · 2018Pooled it
- Environmental Epigenetic Changes, as Risk Factors for the Development of Diseases in Children: A Systematic Review.Annals of global health · 2018Pooled it
- Epigenome-Wide Meta-Analysis of Methylation in Children Related to Prenatal NO2 Air Pollution Exposure.Environmental health perspectives · 2017Pooled it
- The association of exhaled nitric oxide with air pollutants in young infants of asthmatic mothers.Environmental health : a global access science source · 2023Trial
- Effects of Indoor Air Purifiers on Children with Asthma.Yonsei medical journal · 2020Trial
- Reduced mouse allergen is associated with epigenetic changes in regulatory genes, but not mouse sensitization, in asthmatic children.Environmental research · 2017Trial
- Epigenetics: A link between toxicants and diseases.iScience · 2025Review
- Long-term PMPediatric research · 2025Article
- Differences in the DNA methylome of T cells in adults with asthma of varying severity.Clinical epigenetics · 2024Article
- Differences in the DNA Methylome of T cells in Adults With Asthma of Varying Severity.Research square · 2024Article
- Mammographic density and exposure to air pollutants in premenopausal women: a cross-sectional study.Environmental health and preventive medicine · 2024Article
- Associations between mitochondrial biomarkers, urban residential exposures and childhood asthma outcomes over 6 months.Environmental research · 2023Article
- Genetic susceptibility to airway inflammation and exposure to short-term outdoor air pollution.Environmental health : a global access science source · 2023Article
- Ambient Environmental Ozone and Variation of Fractional Exhaled Nitric Oxide (FeNO) in Hairdressers and Healthcare Workers.International journal of environmental research and public health · 2023Article
- Epigenome-wide association of neonatal methylation and trimester-specific prenatal PMEnvironmental epidemiology (Philadelphia, Pa.) · 2022Article
- Emerging Insights into the Impact of Air Pollution on Immune-Mediated Asthma Pathogenesis.Current allergy and asthma reports · 2022Review
- DNA methylation: a potential mediator between air pollution and metabolic syndrome.Clinical epigenetics · 2022Review
- Air pollution-induced epigenetic changes: disease development and a possible link with hypersensitivity pneumonitis.Environmental science and pollution research international · 2021Review
- Exposure to traffic-related air pollution and changes in exhaled nitric oxide and DNA methylation in arginase and nitric oxide synthase in children with asthma.Environmental health : a global access science source · 2021Article
- Epigenetic influence of environmentally neurotoxic metals.Neurotoxicology · 2020Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundInducible nitric oxide synthase (iNOS; encoded by nitric oxide synthase isoform 2 [NOS2]) is the major enzyme for nitric oxide synthesis in airways. As such, measurement of fractional concentration of exhaled nitric oxide (Feno) provides an in vivo assessment of iNOS activity. Short-term exposure to air pollution, haplotypes, and DNA methylation in the NOS2 promoter has been associated independently with iNOS expression, Feno levels, or both.
objectiveWe aimed to examine the effects of ambient air pollutants, NOS2 promoter haplotypes, and NOS2 promoter methylation on Feno levels in children.
methodsWe selected 940 participants in the Children's Health Study who provided buccal samples and had undergone Feno measurement on the same day. DNA methylation was measured with a bisulfite-PCR Pyrosequencing assay. Seven single nucleotide polymorphisms captured the haplotype diversity in the NOS2 promoter. Average particulate matter with an aerodynamic diameter of 2.5 μm or less (PM(2.5)) and 10 μm (PM(10)) or less and ozone and nitrogen dioxide levels 7 days before Feno measurement were estimated based on air pollution data obtained at central monitoring sites.
resultsWe found interrelated effects of PM(2.5), NOS2 promoter haplotypes, and iNOS methylation on Feno levels. Increased 7-day average PM(2.5) exposure was associated with lower iNOS methylation (P = .01). NOS2 promoter haplotypes were globally associated with NOS2 promoter methylation (P = 6.2 × 10(-8)). There was interaction among 1 common promoter haplotype, iNOS methylation level, and PM(2.5) exposure on Feno levels (P(interaction) = .00007).
conclusionPromoter variants in NOS2 and short-term PM(2.5) exposure affect iNOS methylation. This is one of the first studies showing contributions of genetic and epigenetic variations in air pollution-mediated phenotype expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.