ArticleReproductive biology and endocrinology : RB&E2011
The chemokine receptor CXCR4 and its ligand CXCL12 are activated during implantation and placentation in sheep.
Article in Reproductive biology and endocrinology : RB&E, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Trial
- Differences in CXCR4 expression between day 5 and day 6 euploid blastocysts: A predictor of implantation potential in single embryo transfer.Clinical and experimental reproductive medicine · 2026Article
- Involvement of chemokine CXCL12 and its receptor CXCR4 in uterine receptivity and potential relationship to fertility in cattle: a mini review.Frontiers in veterinary science · 2025Review
- Role of chemokines in regulating luteal and uterine functions in pregnant cows.The Journal of reproduction and development · 2024Review
- Placental insufficiency and heavier placentas in sheep after suppressing CXCL12/CXCR4 signaling during implantation†.Biology of reproduction · 2023Article
- Maternal CXCR4 deletion results in placental defects and pregnancy loss mediated by immune dysregulation.JCI insight · 2023Article
- Ruminant conceptus-maternal interactions: interferon-tau and beyond.Journal of animal science · 2022Review
- Chemokine C-X-C receptor 4 mediates recruitment of bone marrow-derived nonhematopoietic and immune cells to the pregnant uterus†.Biology of reproduction · 2022Article
- CXCL12 May Drive Inflammatory Potential in the Ovine Corpus Luteum During Implantation.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Regulation and Function of Chemokines at the Maternal-Fetal Interface.Frontiers in cell and developmental biology · 2022Review
- Inhibition of the C-X-C Motif Chemokine 12 (CXCL12) and Its Receptor CXCR4 Reduces Utero-Placental Expression of the VEGF System and Increases Utero-Placental Autophagy.Frontiers in veterinary science · 2021Article
- Intrauterine inhibition of chemokine receptor 4 signaling modulates local and systemic inflammation in ovine pregnancy.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Chemokines as the modulators of endometrial epithelial cells remodelling.Scientific reports · 2019Article
- CXCR4 signaling at the ovine fetal-maternal interface regulates vascularization, CD34+ cell presence, and autophagy in the endometrium†.Biology of reproduction · 2019Article
- Mechanisms for the establishment and maintenance of pregnancy: synergies from scientific collaborations.Biology of reproduction · 2018Review
- Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.Reproductive biology and endocrinology : RB&E · 2018Article
- In the ovine pituitary, CXCR4 is localized in gonadotropes and somatotropes and increases with elevated serum progesterone.Domestic animal endocrinology · 2018Article
- Characterization of exosomal release in bovine endometrial intercaruncular stromal cells.Reproductive biology and endocrinology : RB&E · 2016Article
- Spontaneous Preterm Delivery, Particularly with Reduced Fetal Growth, is Associated with DNA Hypomethylation of Tumor Related Genes.Journal of pregnancy and child health · 2016Article
- Uterine vasculature remodeling in human pregnancy involves functional macrochimerism by endothelial colony forming cells of fetal origin.Stem cells (Dayton, Ohio) · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe progression of implantation and placentation in ruminants is complex and is regulated by interplay between sex steroids and local signaling molecules, many of which have immune function. Chemokines and their receptors are pivotal factors in implantation and vascularization of the placenta. Based on known critical roles for chemokine receptor 4 (CXCR4) during early pregnancy in other species, we hypothesized that CXCR4 and its ligand CXCL12 would increase in the endometrium and conceptus in response to implantation in ewes. The objectives of the current study were to determine if CXCL12 and CXCR4 were upregulated in: endometrium from pregnant compared to non-pregnant ewes and in, conceptuses, cotyledons, caruncles and intercaruncular tissue.
methodsTissues were collected from sheep on Days 12, 13, 14, and 15 of either the estrous cycle or pregnancy and from pregnant ewes on Days 35 and 50. Blood samples from jugular and uterine vein were also collected on all days. Conceptuses were collected from mature ewes on Days 13, 15, 16, 17, 21 and 30 of gestation. Real time PCR was used to determine relative mRNA concentrations for CXCL12 and CXCR4 and Western blot analysis was employed to confirm protein concentration.
resultsDifferences described are P < 0.05. In the endometrium, CXCR4 mRNA and protein was greater on Day 15 of pregnancy compared to the estrous cycle. CXCL12 and CXCR4 mRNA in conceptuses was greater on Days 21 and 30 compared to earlier days. CXCL12 mRNA was greater in cotyledons on Day 35 compared to Day 50. On Day 35 of gestation, CXCR4 was greater compared to Day 50 in caruncle and intercaruncular tissue. White blood cells obtained from jugular and uterine vein collection had the greatest mRNA concentration of CXCL12 on Day 35 of pregnancy.
conclusionsA comprehensive analysis of CXCL12 and CXCR4 expression in fetal and maternal tissues during early pregnancy is reported with noteworthy differences occurring during implantation and placentation in sheep. We interpreted these data to mean that the CXCL12/CXCR4 pathway is activated during implantation and placentation in sheep and is likely playing a role in the communication between trophoblast cells and the maternal endometrium.
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Registered trials
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