ArticlePloS one2011
Calcium signaling in live cells on elastic gels under mechanical vibration at subcellular levels.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Low-intensity ultrasound induces angiogenesis by activating endothelial integrin signaling in male mice.Physiological reports · 2026Article
- Enhanced Vascularity in Gelatin Scaffolds via Copper-Doped Magnesium-Calcium Silicates Incorporation:ArXiv · 2025Article
- Extracellular bioelectrical lexicon: detecting rhythmic patterns within dermal fibroblast populations.Scientific reports · 2025Article
- Enhanced Vascularity in Gelatin Scaffolds via Copper-Doped Magnesium-Calcium Silicates Incorporation:Ceramics international · 2024Article
- Visualization of Cell Membrane Tension Regulated by the Microfilaments as a "Shock Absorber" in Micropatterned Cells.Biology · 2023Article
- Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.Cell research · 2022Article
- Vibration, a treatment for migraine, linked to calpain driven changes in actin cytoskeleton.PloS one · 2022Article
- Effect of pulsatile stretch on unfolded protein response in a new model of the pulmonary hypertensive vascular wall.Biochemistry and biophysics reports · 2021Article
- Biophysical Tools to Study Cellular Mechanotransduction.Bioengineering (Basel, Switzerland) · 2017Review
- Functional Assay of Cancer Cell Invasion Potential Based on Mechanotransduction of Focused Ultrasound.Frontiers in oncology · 2017Article
- Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP).PloS one · 2016Article
- Rapid and Localized Mechanical Stimulation and Adhesion Assay: TRPM7 Involvement in Calcium Signaling and Cell Adhesion.PloS one · 2015Article
- Non-contact high-frequency ultrasound microbeam stimulation for studying mechanotransduction in human umbilical vein endothelial cells.Ultrasound in medicine & biology · 2014Article
- Cell membrane deformation induced by a fibronectin-coated polystyrene microbead in a 200-MHz acoustic trap.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2014Article
- Prolonged mechanical stretch initiates intracellular calcium oscillations in human mesenchymal stem cells.PloS one · 2014Article
- Local membrane deformation and micro-injury lead to qualitatively different responses in osteoblasts.F1000Research · 2014Article
- Gαq/11-mediated intracellular calcium responses to retrograde flow in endothelial cells.American journal of physiology. Cell physiology · 2012Article
- Attenuation of cell mechanosensitivity in colon cancer cells during in vitro metastasis.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
A new device was designed to generate a localized mechanical vibration of flexible gels where human umbilical vein endothelial cells (HUVECs) were cultured to mechanically stimulate these cells at subcellular locations. A Fluorescence Resonance Energy Transfer (FRET)-based calcium biosensor (an improved Cameleon) was used to monitor the spatiotemporal distribution of intracellular calcium concentrations in the cells upon this mechanical stimulation. A clear increase in intracellular calcium concentrations over the whole cell body (global) can be observed in the majority of cells under mechanical stimulation. The chelation of extracellular calcium with EGTA or the blockage of stretch-activated calcium channels on the plasma membrane with streptomycin or gadolinium chloride significantly inhibited the calcium responses upon mechanical stimulation. Thapsigargin, an endoplasmic reticulum (ER) calcium pump inhibitor, or U73122, a phospholipase C (PLC) inhibitor, resulted in mainly local calcium responses occurring at regions close to the stimulation site. The disruption of actin filaments with cytochalasin D or inhibition of actomyosin contractility with ML-7 also inhibited the global calcium responses. Therefore, the global calcium response in HUVEC depends on the influx of calcium through membrane stretch-activated channels, followed by the release of inositol trisphosphate (IP3) via PLC activation to trigger the ER calcium release. Our newly developed mechanical stimulation device can also provide a powerful tool for the study of molecular mechanism by which cells perceive the mechanical cues at subcellular levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.