ArticleJournal of applied physiology (Bethesda, Md. : 1985)2012
Mild heat stress induces mitochondrial biogenesis in C2C12 myotubes.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 147 citations in OpenAlex.
- Change in Exercise Performance and Markers of Acute Kidney Injury Following Heat Acclimation with Permissive Dehydration.Nutrients · 2021Trial
- Ultrasound-activated piezoelectric muscle constructs for tissue-engineered regenerative peripheral nerve interfaces.Bioactive materials · 2027Article
- Repeated Moderate and Severe Heat Exposure Has Divergent Effects on Skeletal Muscle Mitochondrial, Proteostatic and Neuromuscular Outcomes in Mice.Acta physiologica (Oxford, England) · 2026Article
- Thermal stimulation promotes myogenic differentiation through enhanced glucose uptake and transient lipid droplet accumulation in C2C12 cells.In vitro cellular & developmental biology. Animal · 2026Article
- The Central Role of the AMPK/SIRT1/PGC-1α Signaling Axis in Skeletal Muscle Physiology and Pathology and Its Targeted Therapeutic Strategies.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Heat Therapy: Targeting Health, Disease, and Disability.Comprehensive Physiology · 2026Review
- Skeletal Muscle HSF1 Alleviates Age-Associated Sarcopenia and Mitochondrial Function Decline via SIRT3-PGC1α Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Forty-one degrees celsius enhances proliferation of chicken muscle satellite cells via mechanistic target of rapamycin activation and mitochondrial metabolism.Poultry science · 2025Article
- Mitochondrial adaptations from heat acclimation - A narrative review.Journal of thermal biology · 2025Review
- Bioadhesive and antioxidant hydrogel by MoSMaterials today. Bio · 2025Article
- Vascular, inflammatory and perceptual responses to hot water immersion: Impacts of water depth and temperature in young healthy adults.Experimental physiology · 2025Article
- Exploring mitochondrial dynamics and localization under cellular stress: A comprehensive insight.Journal of biosciences · 2025Review
- Effects and mechanisms of polysaccharides from natural medicinal plants on improving aerobic exercise capacity.Frontiers in nutrition · 2025Review
- Effects of culture temperature (37°C, 39°C) and oxygen concentration (20%, 2%) on proliferation and differentiation of C2C12 cells.Journal of animal science and technology · 2025Article
- The dose-effect response of combined red and infrared photobiomodulation on insulin resistance in skeletal muscle cells.Biochemistry and biophysics reports · 2024Article
- Hot pants: The emerging field of exercise mimetics, from hospital beds to the international space station.Physiological reports · 2024Review
- Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal muscle.Pflugers Archiv : European journal of physiology · 2024Article
- Identification of Molecular Profile of Ear Fibroblasts Derived from Spindle-Transferred Holstein Cattle with Ooplasts from Taiwan Yellow Cattle under Heat Stress.Animals : an open access journal from MDPI · 2024Article
- Heat acclimation improves exercise performance in hot conditions and increases heat shock protein 70 and 90 of skeletal muscles in Thoroughbred horses.Physiological reports · 2024Article
- Inflammatory response in dairy cows caused by heat stress and biological mechanisms for maintaining homeostasis.PloS one · 2024Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
During endurance exercise, most (≈75%) of the energy derived from the oxidation of metabolic fuels and ATP hydrolysis of muscle contraction is liberated as heat, the accumulation of which leads to an increase in body temperature. For example, the temperature of exercising muscles can rise to 40°C. Although severe heat injury can be deleterious, several beneficial effects of mild heat stress (HS), such as the improvement of insulin sensitivity in patients with type 2 diabetes, have been reported. However, among all cellular events induced by mild HS from physical activities, the direct effects and mechanisms of mild HS on mitochondrial biogenesis in skeletal muscle are least characterized. AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1) are key energy-sensing molecules regulating mitochondrial biogenesis. In C2C12 myotubes, we found that 1 h mild HS at 40°C upregulated both AMPK activity and SIRT1 expression, as well as increased the expression of several mitochondrial biogenesis regulatory genes including peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and transcription factors involved in mitochondrial biogenesis. In particular, PGC-1α expression was found to be transcriptionally regulated by mild HS. Additionally, after repeated mild HS for 5 days, protein levels of PGC-1α and several mitochondrial oxidative phosphorylation subunits were also upregulated. Repeated mild HS also significantly increased mitochondrial DNA copy number. In conclusion, these data show that mild HS is sufficient to induce mitochondrial biogenesis in C2C12 myotubes. Temperature-induced mitochondrial biogenesis correlates with activation of the AMPK-SIRT1-PGC-1α pathway. Therefore, it is possible that muscle heat production during exercise plays a role in mitochondrial biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.