ArticleNature protocols2011
Imaging of rat optic nerve axons in vivo.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Retrograde axonal transport of autophagic vesicles and dynein-dynactin protein interaction are attenuated during aging in the rat optic nerve in vivo.Neural regeneration research · 2026Article
- Axonal autophagic vesicle transport in the rat optic nerve in vivo under normal conditions and during acute axonal degeneration.Acta neuropathologica communications · 2024Article
- In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish.Cell regeneration (London, England) · 2021Article
- AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo.Cell death & disease · 2021Article
- Inhibition of the autophagic protein ULK1 attenuates axonal degeneration in vitro and in vivo, enhances translation, and modulates splicing.Cell death and differentiation · 2020Article
- PTEN knockdown with the Y444F mutant AAV2 vector promotes axonal regeneration in the adult optic nerve.Neural regeneration research · 2018Article
- Review
- Attenuation of Axonal Degeneration by Calcium Channel Inhibitors Improves Retinal Ganglion Cell Survival and Regeneration After Optic Nerve Crush.Molecular neurobiology · 2017Article
- Methodological advances in imaging intravital axonal transport.F1000Research · 2017Review
- Tyrosine-mutated AAV2-mediated shRNA silencing of PTEN promotes axon regeneration of adult optic nerve.PloS one · 2017Article
- Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration.Scientific reports · 2016Article
- Alpha-Synuclein affects neurite morphology, autophagy, vesicle transport and axonal degeneration in CNS neurons.Cell death & disease · 2015Article
- ROCK2 is a major regulator of axonal degeneration, neuronal death and axonal regeneration in the CNS.Cell death & disease · 2014Article
- Viral vector-mediated downregulation of RhoA increases survival and axonal regeneration of retinal ganglion cells.Frontiers in cellular neuroscience · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this protocol, we describe the imaging of single axons in the rat optic nerve in vivo. Axons are labeled through the intravitreal injection of adeno-associated viral vectors (AAVs) expressing a fluorophore (duration of the procedure ∼1 h). Two weeks after intravitreal injection, the optic nerve is surgically exposed (duration ∼1 h) and labeled axons are imaged with an epifluorescence microscope either for up to 8 h or repetitively on the following days. Additionally, intravitreal injection of calcium-sensitive dyes allows for imaging of intra-axonal calcium kinetics. This procedure enables the analysis of the morphological changes of degenerating axons in the optic nerve in different lesion paradigms, such as optic nerve crush, axotomy or pin lesion. Furthermore, the effects of pharmacological manipulations on axonal stability and axonal calcium kinetics in axons of the central nervous system can be studied in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.