ArticleNature protocols2011
Multiplexed array-based and in-solution genomic enrichment for flexible and cost-effective targeted next-generation sequencing.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Implementation of a Multicenter Biobanking Collaboration for Next-Generation Sequencing-Based Biomarker Discovery Based on Fresh Frozen Pretreatment Tumor Tissue Biopsies.The oncologist · 2017Trial
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- Identification of candidate genes for developmental colour agnosia in a single unique family.PloS one · 2023Article
- Few-shot learning creates predictive models of drug response that translate from high-throughput screens to individual patients.Nature cancer · 2021Article
- Investigation of Genetic Modifiers of Copper Toxicosis in Labrador Retrievers.Life (Basel, Switzerland) · 2020Article
- A systematic investigation of human DNA preservation in medieval skeletons.Scientific reports · 2020Article
- The role of rare compound heterozygous events in autism spectrum disorder.Translational psychiatry · 2020Article
- The molecular genetic make-up of male breast cancer.Endocrine-related cancer · 2019Article
- Impaired EIF2S3 function associated with a novel phenotype of X-linked hypopituitarism with glucose dysregulation.EBioMedicine · 2019Article
- Inferring the evolution of the major histocompatibility complex of wild pigs and peccaries using hybridisation DNA capture-based sequencing.Immunogenetics · 2018Article
- Role of genomics in promoting the utilization of plant genetic resources in genebanks.Briefings in functional genomics · 2018Review
- A systematic review on the genetics of male infertility in the era of next-generation sequencing.Arab journal of urology · 2018Article
- Predicting clinical benefit from everolimus in patients with advanced solid tumors, the CPCT-03 study.Oncotarget · 2017Article
- Ankyrin repeat and zinc-finger domain-containing 1 mutations are associated with infantile-onset inflammatory bowel disease.The Journal of biological chemistry · 2017Article
- Article
- Joubert syndrome: genotyping a Northern European patient cohort.European journal of human genetics : EJHG · 2016Article
- DNA Methylation Analysis: Choosing the Right Method.Biology · 2016Review
- MKS1 regulates ciliary INPP5E levels in Joubert syndrome.Journal of medical genetics · 2016Article
- Targeted capture in evolutionary and ecological genomics.Molecular ecology · 2016Review
- Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unprecedented increase in the throughput of DNA sequencing driven by next-generation technologies now allows efficient analysis of the complete protein-coding regions of genomes (exomes) for multiple samples in a single sequencing run. However, sample preparation and targeted enrichment of multiple samples has become a rate-limiting and costly step in high-throughput genetic analysis. Here we present an efficient protocol for parallel library preparation and targeted enrichment of pooled multiplexed bar-coded samples. The procedure is compatible with microarray-based and solution-based capture approaches. The high flexibility of this method allows multiplexing of 3-5 samples for whole-exome experiments, 20 samples for targeted footprints of 5 Mb and 96 samples for targeted footprints of 0.4 Mb. From library preparation to post-enrichment amplification, including hybridization time, the protocol takes 5-6 d for array-based enrichment and 3-4 d for solution-based enrichment. Our method provides a cost-effective approach for a broad range of applications, including targeted resequencing of large sample collections (e.g., follow-up genome-wide association studies), and whole-exome or custom mini-genome sequencing projects. This protocol gives details for a single-tube procedure, but scaling to a manual or automated 96-well plate format is possible and discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.