ArticleDevelopmental biology2012
BMP4 signaling directs primitive endoderm-derived XEN cells to an extraembryonic visceral endoderm identity.
Article in Developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 78 citations in OpenAlex.
- Review
- The vertebrate yolk sac: evolutionary origins and current advances in in vitro models.Development (Cambridge, England) · 2026Review
- Synthetic embryos: current progress, application and challenges.Reproductive biology and endocrinology : RB&E · 2026Review
- Primitive means first, not worst: Critical roles for primitive endoderm in embryos and embryo models.Developmental biology · 2025Review
- Single-cell analysis of bidirectional reprogramming between early embryonic states identify mechanisms of differential lineage plasticities in mice.Developmental cell · 2025Article
- Divergent destinies: insights into the molecular mechanisms underlying EPI and PE fate determination.Life science alliance · 2025Review
- Smad4 is essential for epiblast scaling and morphogenesis after implantation, but nonessential before implantation.Development (Cambridge, England) · 2024Article
- OCT4 is expressed in extraembryonic endoderm stem (XEN) cell progenitors during somatic cell reprogramming.bioRxiv : the preprint server for biology · 2024Article
- Automated, High-Throughput Phenotypic Screening and Analysis Platform to Study Pre- and Post-Implantation Morphogenesis in Stem Cell-Derived Embryo-Like Structures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Transcriptional network governing extraembryonic endoderm cell fate choice.Developmental biology · 2023Article
- Dissecting embryonic and extra-embryonic lineage crosstalk with stem cell co-culture.bioRxiv : the preprint server for biology · 2023Article
- The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency.Nature communications · 2023Article
- Journey of the mouse primitive endoderm: from specification to maturation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- The mouse allantois: new insights at the embryonic-extraembryonic interface.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Stem-cell-based human and mouse embryo models.Current opinion in genetics & development · 2022Review
- Stress Decreases Host Viral Resistance and Increases Covid Susceptibility in Embryonic Stem Cells.Stem cell reviews and reports · 2021Article
- Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States.International journal of molecular sciences · 2021Article
- Role of Iron Metabolism-Related Genes in Prenatal Development: Insights from Mouse Transgenic Models.Genes · 2021Review
- All models are wrong, but some are useful: Establishing standards for stem cell-based embryo models.Stem cell reports · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The visceral endoderm (VE) is an epithelial tissue in the early postimplantation mouse embryo that encapsulates the pluripotent epiblast distally and the extraembryonic ectoderm proximally. In addition to facilitating nutrient exchange before the establishment of a circulation, the VE is critical for patterning the epiblast. Since VE is derived from the primitive endoderm (PrE) of the blastocyst, and PrE-derived eXtraembryonic ENdoderm (XEN) cells can be propagated in vitro, XEN cells should provide an important tool for identifying factors that direct VE differentiation. In this study, we demonstrated that BMP4 signaling induces the formation of a polarized epithelium in XEN cells. This morphological transition was reversible, and was associated with the acquisition of a molecular signature comparable to extraembryonic (ex) VE. Resembling exVE which will form the endoderm of the visceral yolk sac, BMP4-treated XEN cells regulated hematopoiesis by stimulating the expansion of primitive erythroid progenitors. We also observed that LIF exerted an antagonistic effect on BMP4-induced XEN cell differentiation, thereby impacting the extrinsic conditions used for the isolation and maintenance of XEN cells in an undifferentiated state. Taken together, our data suggest that XEN cells can be differentiated towards an exVE identity upon BMP4 stimulation and therefore represent a valuable tool for investigating PrE lineage differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.