ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Opposing roles of voltage-gated Ca2+ channels in neuronal control of regenerative patterning.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 50 citations in OpenAlex.
- Conserved innate immunity components limit transgene expression in adult planarians.Development (Cambridge, England) · 2026Article
- Praziquantel Pretreatment ReducesTropical medicine and infectious disease · 2025Article
- Unveiling an ancient whole-genome duplication event in Stentor, the model unicellular eukaryotes.Science China. Life sciences · 2025Article
- Electrophysiological characterization of a schistosome transient receptor potential channel activated by praziquantel.International journal for parasitology · 2023Article
- Natural variation in the binding pocket of a parasitic flatworm TRPM channel resolves the basis for praziquantel sensitivity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A Wnt11 and Dishevelled signaling pathway acts prior to injury to control wound polarization for the onset of planarian regeneration.Current biology : CB · 2022Article
- Localized electrical stimulation triggers cell-type-specific proliferation in biofilms.Cell systems · 2022Article
- The feedback loop between calcineurin, calmodulin-dependent protein kinase II, and nuclear factor of activated T-cells regulates the number of GABAergic neurons during planarian head regeneration.Frontiers in molecular neuroscience · 2022Article
- Direct Current Electric Stimulation Alters the Frequency and the Distribution of Mitotic Cells in Planarians.Bioelectricity · 2021Article
- Integrating Bioelectrical Currents and CaBioelectricity · 2020Review
- Activation of transient receptor potential channel Sm.(Schistosoma mansoni)TRPMParasitology research · 2020Review
- The Journey to Discovering a Flatworm Target of Praziquantel: A Long TRP.Trends in parasitology · 2020Review
- Cortex Mori Radicis extract promotes neurite outgrowth in diabetic rats by activating PI3K/AKT signaling and inhibiting Ca2+ influx associated with the upregulation of transient receptor potential canonical channel 1.Molecular medicine reports · 2020Article
- CaCold Spring Harbor perspectives in biology · 2019Review
- Planarian regeneration as a model of anatomical homeostasis: Recent progress in biophysical and computational approaches.Seminars in cell & developmental biology · 2019Review
- Live imaging of intracellular pH in planarians using the ratiometric fluorescent dye SNARF-5F-AM.Biology methods & protocols · 2019Article
- Activation of host transient receptor potential (TRP) channels by praziquantel stereoisomers.PLoS neglected tropical diseases · 2018Article
- Endogenous Bioelectric Signaling Networks: Exploiting Voltage Gradients for Control of Growth and Form.Annual review of biomedical engineering · 2017Review
- Utilizing the planarian voltage-gated ion channel transcriptome to resolve a role for a CaBiochimica et biophysica acta. Molecular cell research · 2017Article
- Planarian regeneration in space: Persistent anatomical, behavioral, and bacteriological changes induced by space travel.Regeneration (Oxford, England) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
There is intense interest in developing methods to regulate proliferation and differentiation of stem cells into neuronal fates for the purposes of regenerative medicine. One way to do this is through in vivo pharmacological engineering using small molecules. However, a key challenge is identification of relevant signaling pathways and therein druggable targets to manipulate stem cell behavior efficiently in vivo. Here, we use the planarian flatworm as a simple chemical-genetic screening model for nervous system regeneration to show that the isoquinoline drug praziquantel (PZQ) acts as a small molecule neurogenic to produce two-headed animals with integrated CNSs following regeneration. Characterization of the entire family of planarian voltage-operated Ca(2+) channel α subunits (Ca(v)α), followed by in vivo RNAi of specific Ca(v) subunits, revealed that PZQ subverted regeneration by activation of a specific voltage-gated Ca(2+) channel isoform (Ca(v)1A). PZQ-evoked Ca(2+) entry via Ca(v)1A served to inhibit neuronally derived Hedgehog signals, as evidenced by data showing that RNAi of Ca(v)1A prevented PZQ-evoked bipolarity, Ca(2+) entry, and decreases in wnt1 and wnt11-5 levels. Surprisingly, the action of PZQ was opposed by Ca(2+) influx through a closely related neuronal Ca(v) isoform (Ca(v)1B), establishing a novel interplay between specific Ca(v)1 channel isoforms, Ca(2+) entry, and neuronal Hedgehog signaling. These data map PZQ efficacy to specific neuronal Ca(v) complexes in vivo and underscore that both activators (Ca(v)1A) and inhibitors (Ca(v)1B) of Ca(2+) influx can act as small molecule neurogenics in vivo on account of the unique coupling of Ca(2+) channels to neuronally derived polarity cues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.