ArticleThe Journal of biological chemistry2012
Loading-related regulation of transcription factor EGR2/Krox-20 in bone cells is ERK1/2 protein-mediated and prostaglandin, Wnt signaling pathway-, and insulin-like growth factor-I axis-dependent.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
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- Deletion of TECRL promotes skeletal muscle repair by up-regulating EGR2.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Deciphering Master Gene Regulators and Associated Networks of Human Mesenchymal Stromal Cells.Biomolecules · 2020Article
- Modulation of bone turnover aberration: A target for management of primary osteoporosis in experimental rat model.Heliyon · 2020Article
- Comparative Transcriptome Analysis of the Regenerating Zebrafish Telencephalon Unravels a Resource With Key Pathways During Two Early Stages and Activation of Wnt/β-Catenin Signaling at the Early Wound Healing Stage.Frontiers in cell and developmental biology · 2020Article
- A G protein-coupled, IP3/protein kinase C pathway controlling the synthesis of phosphaturic hormone FGF23.JCI insight · 2019Article
- Towards a gene regulatory network shaping the fins of the Princess cichlid.Scientific reports · 2018Article
- Regulation of early growth response 2 expression by secreted frizzled related protein 1.BMC cancer · 2017Article
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- Transcription factor ZNF25 is associated with osteoblast differentiation of human skeletal stem cells.BMC genomics · 2016Article
- The Essentiality of Arachidonic Acid in Infant Development.Nutrients · 2016Review
- Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing.Histochemistry and cell biology · 2016Article
- Identification of Differentially Expressed Genes Associated with Litter Size in Berkshire Pig Placenta.PloS one · 2016Article
- Preclinical models for in vitro mechanical loading of bone-derived cells.BoneKEy reports · 2015Article
- Wnt16 Is Associated with Age-Related Bone Loss and Estrogen Withdrawal in Murine Bone.PloS one · 2015Article
- Protein kinase Cα (PKCα) regulates bone architecture and osteoblast activity.The Journal of biological chemistry · 2014Article
- Age-related impairment of bones' adaptive response to loading in mice is associated with sex-related deficiencies in osteoblasts but no change in osteocytes.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- The anti-diabetic drug metformin does not affect bone mass in vivo or fracture healing.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2013Article
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9 authors at 3 institutions in 2 countries.
Funding
Abstract
Of the 1,328 genes revealed by microarray to be differentially regulated by disuse, or at 8 h following a single short period of osteogenic loading of the mouse tibia, analysis by predicting associated transcription factors from annotated affinities revealed the transcription factor EGR2/Krox-20 as being more closely associated with more pathways and functions than any other. Real time quantitative PCR confirmed up-regulation of Egr2 mRNA expression by loading of the tibia in vivo. In vitro studies where strain was applied to primary cultures of mouse tibia-derived osteoblastic cells and the osteoblast UMR106 cell line also showed up-regulation of Egr2 mRNA expression. In UMR106 cells, inhibition of β1/β3 integrin function had no effect on strain-related Egr2 expression, but it was inhibited by a COX2-selective antagonist and imitated by exogenous prostaglandin E2 (PGE2). This response to PGE(2) was mediated chiefly through the EP1 receptor and involved stimulation of PKC and attenuation by cAMP/PKA. Neither activators nor inhibitors of nitric oxide, estrogen signaling, or LiCl had any effect on Egr2 mRNA expression, but it was increased by both insulin-like growth factor-1 and high, but not low, dose parathyroid hormone and exogenous Wnt-3a. The increases by strain, PGE2, Wnt-3a, and phorbol 12-myristate 13-acetate were attenuated by inhibition of MEK-1. EGR2 appears to be involved in many of the signaling pathways that constitute early responses of bone cells to strain. These pathways all have multiple functions. Converting their strain-related responses into coherent "instructions" for adaptive (re)modeling is likely to depend upon their contextual activation, suppression, and interaction probably on more than one occasion.
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