ArticleMolecular biology of the cell2012
Several RNase T2 enzymes function in induced tRNA and rRNA turnover in the ciliate Tetrahymena.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 66 citations in OpenAlex.
- Comparative genomics reveals LINE-1 recombination with diverse RNAs.Cell genomics · 2026Article
- tRNA-derived RNA processing in sperm transmits non-genetically inherited phenotypes to offspring in C. elegans.Nature communications · 2026Article
- Phosphoglycerate dehydrogenase is required for kernel development and defines a predominant serine synthesis pathway in maize.The Plant cell · 2025Article
- tRF-Pro-CGG Suppresses Cell Proliferation and Promotes Apoptosis in Pancreatic Cancer.Digestive diseases and sciences · 2025Article
- Article
- Epididymis-specific RNase A family genes regulate fertility and small RNA processing.The Journal of biological chemistry · 2024Article
- Epididymis-specific RNase A family genes regulate fertility and small RNA processing.bioRxiv : the preprint server for biology · 2024Article
- Multiple regulatory roles of the transfer RNA-derived small RNAs in cancers.Genes & diseases · 2024Review
- tRNA-derived small RNAs (tsRNAs): establishing their dominance in the regulation of human cancer.Frontiers in genetics · 2024Review
- The life and times of a tRNA.RNA (New York, N.Y.) · 2023Review
- Sperm RNA Payload: Implications for Intergenerational Epigenetic Inheritance.International journal of molecular sciences · 2023Review
- Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs.Nucleic acids research · 2023Article
- tRNA-derived small RNAs in plant response to biotic and abiotic stresses.Frontiers in plant science · 2023Review
- tRNA derived fragments:A novel player in gene regulation and applications in cancer.Frontiers in oncology · 2023Review
- tiRNAs: Insights into Their Biogenesis, Functions, and Future Applications in Livestock Research.Non-coding RNA · 2022Review
- tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight.BMC plant biology · 2022Article
- Genome-wide identification and characterization of legume3 Biotech · 2021Article
- Origins and evolving functionalities of tRNA-derived small RNAs.Trends in biochemical sciences · 2021Review
- PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications.Nature cell biology · 2021Article
- tRNA-derived fragments (tRFs): establishing their turf in post-transcriptional gene regulation.Cellular and molecular life sciences : CMLS · 2021Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNase T2 enzymes are produced by a wide range of organisms and have been implicated to function in diverse cellular processes, including stress-induced anticodon loop cleavage of mature tRNAs to generate tRNA halves. Here we describe a family of eight RNase T2 genes (RNT2A-RNT2H) in the ciliate Tetrahymena thermophila. We constructed strains lacking individual or combinations of these RNT2 genes that were viable but had distinct cellular and molecular phenotypes. In strains lacking only one Rnt2 protein or lacking a subfamily of three catalytically inactive Rnt2 proteins, starvation-induced tRNA fragments continued to accumulate, with only a minor change in fragment profile in one strain. We therefore generated strains lacking pairwise combinations of the top three candidates for Rnt2 tRNases. Each of these strains showed a distinct starvation-specific profile of tRNA and rRNA fragment accumulation. These results, the delineation of a broadened range of conditions that induce the accumulation of tRNA halves, and the demonstration of a predominantly ribonucleoprotein-free state of tRNA halves in cell extract suggest that ciliate tRNA halves are degradation intermediates in an autophagy pathway induced by growth arrest that functions to recycle idle protein synthesis machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.