ArticleMolecular and cellular biology1990
Regulation of tubulin levels and microtubule assembly in Saccharomyces cerevisiae: consequences of altered tubulin gene copy number.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 128 citations in OpenAlex.
- Comparative modeling reveals the molecular determinants of aneuploidy fitness cost in a wild yeast model.Cell genomics · 2024Article
- Comparative modeling reveals the molecular determinants of aneuploidy fitness cost in a wild yeast model.bioRxiv : the preprint server for biology · 2024Article
- Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin.The Journal of cell biology · 2023Article
- A distinct P-body-like granule is induced in response to the disruption of microtubule integrity in Saccharomyces cerevisiae.Genetics · 2022Article
- Microtubules in Microorganisms: How Tubulin Isotypes Contribute to Diverse Cytoskeletal Functions.Frontiers in cell and developmental biology · 2022Review
- Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis.The Journal of cell biology · 2021Article
- Multilayered regulation of proteome stoichiometry.Current genetics · 2021Review
- Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.Molecular biology of the cell · 2021Article
- Expression attenuation as a mechanism of robustness against gene duplication.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules.Frontiers in plant science · 2020Article
- Article
- The role of protein complexes in human genetic disease.Protein science : a publication of the Protein Society · 2019Review
- Why haploinsufficiency persists.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Selective defects in gene expression control genome instability in yeast splicing mutants.Molecular biology of the cell · 2019Article
- The TOG protein Stu2/XMAP215 interacts covalently and noncovalently with SUMO.Cytoskeleton (Hoboken, N.J.) · 2018Article
- TheJournal of developmental biology · 2017Article
- Tau-based fluorescent protein fusions to visualize microtubules.Cytoskeleton (Hoboken, N.J.) · 2017Article
- The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells.Scientific reports · 2017Article
- Aneuploidy Causes Non-genetic Individuality.Cell · 2017Article
- Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubule organization in the cytoplasm is in part a function of the number and length of the assembled polymers. The intracellular concentration of tubulin could specify those parameters. Saccharomyces cerevisiae strains constructed with moderately decreased or increased copy numbers of tubulin genes provide an opportunity to study the cellular response to a steady-state change in tubulin concentration. We found no evidence of a mechanism for adjusting tubulin concentrations upward from a deficit, nor did we find a need for such a mechanism: cells with no more than 50% of the wild-type tubulin level were normal with respect to a series of microtubule-dependent properties. Strains with increased copies of both alpha- and beta-tubulin genes, or of alpha-tubulin genes alone, apparently did down regulate their tubulin levels. As a result, they contained greater than normal concentrations of tubulin but much less than predicted from the increase in gene number. Some of this down regulation occurred at the level of protein. These strains were also phenotypically normal. Cells could contain excess alpha-tubulin protein without detectable consequences, but perturbations resulting in excess beta-tubulin genes may have affected microtubule-dependent functions. All of the observed regulation of levels of tubulin can be explained as a response to toxicity associated with excess tubulin proteins, especially if beta-tubulin is much more toxic than alpha-tubulin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.