ArticleBMC genomics2011
Transcriptome analysis of Bupleurum chinense focusing on genes involved in the biosynthesis of saikosaponins.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 93 citations in OpenAlex.
- Proteomic profiling reveals the mechanism of ginsenoside synthesis inJournal of ginseng research · 2026Article
- Heterosis unveiled in root-related traits and saikosaponins content between triploid FFrontiers in plant science · 2026Article
- Multi-strategy ugt mining, modification and glycosyl donor synthesis facilitate the production of triterpenoid saponins.Frontiers in plant science · 2025Review
- Integrated metabolomics and transcriptomics analysis of roots of Bupleurum chinense and B. scorzonerifolium, two sources of medicinal Chaihu.Scientific reports · 2022Article
- The tomato cytochrome P450 CYP712G1 catalyses the double oxidation of orobanchol en route to the rhizosphere signalling strigolactone, solanacol.The New phytologist · 2022Article
- Drought Stress Stimulates the Terpenoid Backbone and Triterpenoid Biosynthesis Pathway to Promote the Synthesis of Saikosaponin inMolecules (Basel, Switzerland) · 2022Article
- Chromosome-Level Genome Assembly ofFrontiers in genetics · 2022Article
- Regulatory network of ginsenoside biosynthesis under Ro stress in the hairy roots ofFrontiers in bioengineering and biotechnology · 2022Article
- Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense.BMC genomics · 2021Article
- Impact of Genomic and Transcriptomic Resources on Apiaceae Crop Breeding Strategies.International journal of molecular sciences · 2021Review
- Analysis of unigenes involved in lateral root development in Bupleurum chinense and B. scorzonerifolium.Planta · 2021Article
- Bayogenin 3-O-cellobioside confers non-cultivar-specific defence against the rice blast fungus Pyricularia oryzae.Plant biotechnology journal · 2021Article
- Transcriptomic and Metabolomic Differences Between TwoFrontiers in bioengineering and biotechnology · 2021Article
- Differential Expression of Genes Involved in Saikosaponin Biosynthesis BetweenFrontiers in genetics · 2020Article
- Effects of drought-re-watering-drought on the photosynthesis physiology and secondary metabolite production of Bupleurum chinense DC.Plant cell reports · 2019Article
- A comprehensive review and perspectives on pharmacology and toxicology of saikosaponins.Phytomedicine : international journal of phytotherapy and phytopharmacology · 2018Review
- The Role of Saikosaponins in Therapeutic Strategies for Age-Related Diseases.Oxidative medicine and cellular longevity · 2018Review
- Comparative transcriptome analysis of shoot and root tissue of Bacopa monnieri identifies potential genes related to triterpenoid saponin biosynthesis.BMC genomics · 2017Article
- Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera.Scientific reports · 2016Article
- Anti-Inflammation Effects and Potential Mechanism of Saikosaponins by Regulating Nicotinate and Nicotinamide Metabolism and Arachidonic Acid Metabolism.Inflammation · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. The 454 pyrosequencing technology provides a promising opportunity for finding novel genes that participate in plant metabolism. Consequently, this technology may help to identify the candidate genes involved in the saikosaponin biosynthetic pathway.
resultsOne-quarter of the 454 pyrosequencing runs produced a total of 195, 088 high-quality reads, with an average read length of 356 bases (NCBI SRA accession SRA039388). A de novo assembly generated 24, 037 unique sequences (22, 748 contigs and 1, 289 singletons), 12, 649 (52.6%) of which were annotated against three public protein databases using a basic local alignment search tool (E-value ≤1e-10). All unique sequences were compared with NCBI expressed sequence tags (ESTs) (237) and encoding sequences (44) from the Bupleurum genus, and with a Sanger-sequenced EST dataset (3, 111). The 23, 173 (96.4%) unique sequences obtained in the present study represent novel Bupleurum genes. The ESTs of genes related to saikosaponin biosynthesis were found to encode known enzymes that catalyze the formation of the saikosaponin backbone; 246 cytochrome P450 (P450s) and 102 glycosyltransferases (GTs) unique sequences were also found in the 454 dataset. Full length cDNAs of 7 P450s and 7 uridine diphosphate GTs (UGTs) were verified by reverse transcriptase polymerase chain reaction or by cloning using 5' and/or 3' rapid amplification of cDNA ends. Two P450s and three UGTs were identified as the most likely candidates involved in saikosaponin biosynthesis. This finding was based on the coordinate up-regulation of their expression with β-AS in methyl jasmonate-treated adventitious roots and on their similar expression patterns with β-AS in various B. chinense tissues.
conclusionsA collection of high-quality ESTs for B. chinense obtained by 454 pyrosequencing is provided here for the first time. These data should aid further research on the functional genomics of B. chinense and other Bupleurum species. The candidate genes for enzymes involved in saikosaponin biosynthesis, especially the P450s and UGTs, that were revealed provide a substantial foundation for follow-up research on the metabolism and regulation of the saikosaponins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.