ArticlePloS one2011
A Brassica napus lipase locates at the membrane contact sites involved in chloroplast development.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Binary division of plastids involves endoplasmic reticulum mediation.Journal of experimental botany · 2025Article
- Review
- Proximity driven plastid-nucleus relationships are facilitated by tandem plastid-ER dynamics.Journal of experimental botany · 2024Article
- ER-associated VAP27-1 and VAP27-3 proteins functionally link the lipid-binding ORP2A at the ER-chloroplast contact sites.Nature communications · 2024Article
- Plastid Transient and Stable Interactions with Other Cell Compartments.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024Review
- Integrated Analysis of Metabolome and Transcriptome Revealed Different Regulatory Networks of Metabolic Flux in Tea Plants [International journal of molecular sciences · 2023Article
- Co-regulation of mitochondrial and chloroplast function: Molecular components and mechanisms.Plant communications · 2023Review
- Membrane contacts with the endoplasmic reticulum modulate plastid morphology and behaviour.Frontiers in plant science · 2023Article
- Membrane contact sites and cytoskeleton-membrane interactions in autophagy.FEBS letters · 2022Review
- The Passage of HPlants (Basel, Switzerland) · 2022Review
- Organelle extensions in plant cells.Plant physiology · 2021Article
- A Combinatorial Reporter Set to Visualize the Membrane Contact Sites Between Endoplasmic Reticulum and Other Organelles in Plant Cell.Frontiers in plant science · 2020Article
- Communications Between the Endoplasmic Reticulum and Other Organelles During Abiotic Stress Response in Plants.Frontiers in plant science · 2019Review
- Lipid Trafficking at Membrane Contact Sites During Plant Development and Stress Response.Frontiers in plant science · 2019Article
- Lipid transport required to make lipids of photosynthetic membranes.Photosynthesis research · 2018Review
- ER: the Silk Road of interorganellar communication.Current opinion in plant biology · 2018Review
- Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules.Frontiers in plant science · 2018Article
- Advances in Plant ER Architecture and Dynamics.Plant physiology · 2018Review
- Chloroplast signaling within, between and beyond cells.Frontiers in plant science · 2015Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFatty acids synthesized in chloroplast are transported to endoplasmic reticulum (ER) for triacylglycerols (TAGs) resembling. The development of chloroplast also requires lipids trafficking from ER to chloroplast. The membrane contact sites (MCSs) between ER and chloroplast has been demonstrated to be involved for the trafficking of lipids and proteins. Lipids trafficking between ER and chloroplast is often accompanied by lipids interconversion. However, it is rarely known how lipids interconversion happens during their trafficking. METHODOLOGY/PRINCIPAL
findingsWe cloned a lipase gene from Brassica napus L., designated as BnCLIP1. Green fluorescence protein (GFP)-tagged BnCLIP1 was shown to locate at the MCSs between ER and chloroplasts in tobacco leaves. Heterogeneous expression of BnCLIP1 in Saccharomyces cerevisiae (pep4) reduced the total amount of fatty acid. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the truncated BnCLIP1 had a substrate preference for C16:0 lipids in Saccharomyces cerevisiae (pep4). To probe the physiological function of BnCLIP1, two Brassica napus lines with different oil-content were introduced to investigate the transcript patterns of BnCLIP1 during seed development. Intriguingly, the transcript level of BnCLIP1 was found to be immediately up-regulated during the natural seed senescence of both lines; the transcription response of BnCLIP1 in the high oil-content seeds was faster than the lower ones, suggesting a potential role of BnCLIP1 in affecting seed oil synthesis via regulating chloroplast integrity. Further researches showed that chemical disruption of leaf chloroplast also activated the transcription of BnCLIP1. CONCLUSIONS/SIGNIFICANCE: The findings of this study show that BnCLIP1 encodes a lipase, localizes at the MCSs and involves in chloroplast development.
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