ArticlePloS one2011
Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- Antibacterial potential of five phages in controlling Enterococcus faecalis and Enterococcus faecium.Virology journal · 2026Article
- Directed evolution of phages in biofilms enhances Pseudomonas aeruginosa control through improved lipopolysaccharide recognition.Nature communications · 2025Article
- Amino acid residues in the tail fiber differentiate the host specificity ofJournal of virology · 2025Article
- Characterization of phage evolution and phage resistance in drug-resistantJournal of virology · 2024Article
- Bacteriolytic Potential ofViruses · 2023Article
- Phenotypic Characterization and Comparative Genomic Analysis of NovelInternational journal of molecular sciences · 2023Article
- Evolutionary Dynamics between Phages and Bacteria as a Possible Approach for Designing Effective Phage Therapies against Antibiotic-Resistant Bacteria.Antibiotics (Basel, Switzerland) · 2022Review
- In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success.Viruses · 2022Review
- Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control.Environmental microbiology · 2022Article
- Intracameral Bacteriophage Injection as Postoperative Prophylaxis for Enterococcus faecalis-Induced Endophthalmitis After Cataract Surgery in Rabbits.Translational vision science & technology · 2022Article
- Genomic characterization of a new phage BUCT541 againstFrontiers in microbiology · 2022Article
- Characterization of the Bacteriophage BUCT603 and Therapeutic Potential Evaluation Against Drug-ResistantFrontiers in microbiology · 2022Article
- Improved bactericidal efficacy and thermostability of Staphylococcus aureus-specific bacteriophage SA3821 by repeated sodium pyrophosphate challenges.Scientific reports · 2021Article
- Biological characteristics and anti-biofilm activity of a lytic phage against vancomycin-resistantIranian journal of microbiology · 2021Article
- Clostridioides difficile phage biology and application.FEMS microbiology reviews · 2021Review
- In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment ofMicroorganisms · 2021Article
- A continuously changing selective context on microbial communities associated with fish, from egg to fork.Evolutionary applications · 2020Article
- Therapeutic Effects of Intravitreously Administered Bacteriophage in a Mouse Model of Endophthalmitis Caused by Vancomycin-Sensitive or -Resistant Enterococcus faecalis.Antimicrobial agents and chemotherapy · 2019Article
- Article
- Unravelling the Links between Phage Adsorption and Successful Infection in Clostridium difficile.Viruses · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some bacterial strains of the multidrug-resistant Gram-positive bacteria Enterococcus faecalis can significantly reduce the efficacy of conventional antimicrobial chemotherapy. Thus, the introduction of bacteriophage (phage) therapy is expected, where a phage is used as a bioagent to destroy bacteria. E. faecalis phage ΦEF24C is known to be a good candidate for a therapeutic phage against E. faecalis. However, this therapeutic phage still produces nonuniform antimicrobial effects with different bacterial strains of the same species and this might prove detrimental to its therapeutic effects. One solution to this problem is the preparation of mutant phages with higher activity, based on a scientific rationale. This study isolated and analyzed a spontaneous mutant phage, ΦEF24C-P2, which exhibited higher infectivity against various bacterial strains when compared with phage ΦEF24C. First, the improved bactericidal effects of phage ΦEF24C-P2 were attributable to its increased adsorption rate. Moreover, genomic sequence scanning revealed that phage ΦEF24C-P2 had a point mutation in orf31. Proteomic analysis showed that ORF31 (mw, 203 kDa) was present in structural components, and immunological analysis using rabbit-derived antibodies showed that it was a component of a long, flexible fine tail fiber extending from the tail end. Finally, phage ΦEF24C-P2 also showed higher bactericidal activity in human blood compared with phage ΦEF24C using the in vitro assay system. In conclusion, the therapeutic effects of phage ΦEF24C-P2 were improved by a point mutation in gene orf31, which encoded a tail fiber component.
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