ArticleDiabetes & vascular disease research2012
Citrate treatment reduces endothelial death and inflammation under hyperglycaemic conditions.
Article in Diabetes & vascular disease research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03319680 (Prospective Randomized Multicenter Study to Demonstrate the Benefits of Hemodialysis Without Acetate), which is not on this map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Prospective Randomized Multicenter Study to Demonstrate the Benefits of Hemodialysis Without Acetate (With Citrate): ABC-treat Study
Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Citrate high volume on-line hemodiafiltration modulates serum Interleukin-6 and Klotho levels: the multicenter randomized controlled study "Hephaestus".Journal of nephrology · 2021Trial
- Lime powder treatment reduces urinary excretion of total protein and transferrin but increases uromodulin excretion in patients with urolithiasis.Urolithiasis · 2018Trial
- Replacement of acetate with citrate in dialysis fluid: a randomized clinical trial of short term safety and fluid biocompatibility.BMC nephrology · 2013Trial
- Post-dilution on line haemodiafiltration with citrate dialysate: first clinical experience in chronic dialysis patients.TheScientificWorldJournal · 2013Trial
- PFOA induced metabolic and immune perturbations in a SARS-2 infection model.bioRxiv : the preprint server for biology · 2026Article
- Effect of dialysate buffer practices on serum parathyroid hormone concentrations in real-life french patients receiving hemodialysis.PloS one · 2026Observational
- Impact of citrate dialysate on middle molecular weight uremic toxin adsorption and removal in high-flux and MCO dialysis membranes.Scientific reports · 2025Article
- Metabolomic differences between exanthematous drug eruption and infectious mononucleosis.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- Analysis of the urinary metabolic profiles in irradiated rats treated with Activated Protein C (APC), a potential mitigator of radiation toxicity.International journal of radiation biology · 2023Article
- Review
- Impact of Acetate versus Citrate Dialysates on Intermediary Metabolism-A Targeted Metabolomics Approach.International journal of molecular sciences · 2022Article
- The metabolomic differential plasma profile between dialysates. Pursuing to understand the mechanisms of citrate dialysate clinical benefits.Frontiers in physiology · 2022Article
- A high magnesium concentration in citrate dialysate prevents oxidative stress and damage in human monocytesClinical kidney journal · 2021Article
- Impact of anion exchange adsorbents on regional citrate anticoagulation.The International journal of artificial organs · 2021Article
- Citrate reduced oxidative damage in stem cells by regulating cellular redox signaling pathways and represent a potential treatment for oxidative stress-induced diseases.Redox biology · 2019Article
- Citrate chemistry and biology for biomaterials design.Biomaterials · 2018Review
- Regional citrate anticoagulation for renal replacement therapies in patients with acute kidney injury: a position statement of the Work Group "Renal Replacement Therapies in Critically Ill Patients" of the Italian Society of Nephrology.Journal of nephrology · 2015Review
- Regional citrate anticoagulation for RRTs in critically ill patients with AKI.Clinical journal of the American Society of Nephrology : CJASN · 2014Review
- Endothelial dysfunction and renal fibrosis in endotoxemia-induced oliguric kidney injury: possible role of LPS-binding protein.Critical care (London, England) · 2014Article
- Citric acid effects on brain and liver oxidative stress in lipopolysaccharide-treated mice.Journal of medicinal food · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyperglycaemia and glucose degradation products (GDPs) are closely associated with oxidative stress and inflammation in diabetic patients, a condition that leads to endothelial dysfunction and cardiovascular problems. We evaluated the effect of citrate and gluconate on glucose- and GDP-induced endothelial inflammation by measuring changes in viability, inflammation and function in primary human umbilical vein endothelial cells (HUVECs). The extent of apoptosis/necrosis was measured by flow cytometry and visualised with confocal microscopy by staining with annexin V or propidium iodide, respectively. Protein kinase C-βII (PKC-βII) activation was evaluated with Western blotting. Incubation with glucose (30 mM) and GDP (50 µM) significantly increased PKC-βII expression, endothelial cell death and inflammation. The addition of citrate decreased hyperglycaemia-induced apoptosis (p = 0.021), necrosis (p = 0.04) and reduced PKC-βII expression (p = 0.021) down to background levels. Citrate improved endothelial function by reducing the inflammatory markers (p = 0.01) and by decreasing neutrophil diapedesis (p = 0.012). These results suggest that citrate may have therapeutic potential by reducing hyperglycaemia-induced endothelial inflammation and abolishing endothelial dysfunction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.