ArticleJournal of cell science2011
Ultrastructural study of transcription factories in mouse erythroblasts.
Article in Journal of cell science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- RNA polymerase II transcription compartments - from factories to condensates.Nature reviews. Genetics · 2025Review
- Revisiting models of enhancer-promoter communication in gene regulation.Genome research · 2025Review
- Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture.Cold Spring Harbor perspectives in biology · 2022Review
- Live imaging of transcription sites using an elongating RNA polymerase II-specific probe.The Journal of cell biology · 2022Article
- Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches.Nucleic acids research · 2022Article
- Spatial organization of transcribing loci during early genome activation in Drosophila.Current biology : CB · 2021Article
- The dynamic broad epigenetic (H3K4me3, H3K27ac) domain as a mark of essential genes.Clinical epigenetics · 2021Review
- Cellular dynamics of mammalian red blood cell production in the erythroblastic island niche.Biophysical reviews · 2019Review
- Cajal body function in genome organization and transcriptome diversity.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Spatially coordinated replication and minimization of expression noise constrain three-dimensional organization of yeast genome.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Morphology of nuclear transcription.Histochemistry and cell biology · 2016Review
- Dissecting the nascent human transcriptome by analysing the RNA content of transcription factories.Nucleic acids research · 2015Article
- Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei.Journal of experimental botany · 2015Article
- Co-expressed genes prepositioned in spatial neighborhoods stochastically associate with SC35 speckles and RNA polymerase II factories.Cellular and molecular life sciences : CMLS · 2014Article
- Chromatin immunoprecipitation indirect peaks highlight long-range interactions of insulator proteins and Pol II pausing.Molecular cell · 2014Article
- Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Space exploration by the promoter of a long human gene during one transcription cycle.Nucleic acids research · 2013Article
- EKLF/KLF1, a tissue-restricted integrator of transcriptional control, chromatin remodeling, and lineage determination.Molecular and cellular biology · 2013Review
- Correlative fluorescence and EFTEM imaging of the organized components of the mammalian nucleus.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Novel roles for KLF1 in erythropoiesis revealed by mRNA-seq.Genome research · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RNA polymerase II (RNAPII) transcription has been proposed to occur at transcription factories; nuclear focal accumulations of the active, phosphorylated forms of RNAPII. The low ratio of transcription factories to active genes and transcription units suggests that genes must share factories. Our previous analyses using light microscopy have indicated that multiple genes could share the same factory. Furthermore, we found that a small number of specialized transcription factories containing high levels of the erythroid-specific transcription factor KLF1 preferentially transcribed a network of KLF1-regulated genes. Here we used correlative light microscopy in combination with energy filtering transmission electron microscopy (EFTEM) and electron microscopy in situ hybridization (EMISH) to analyse transcription factories, transcribing genes, and their nuclear environments at the ultrastructural level in ex vivo mouse foetal liver erythroblasts. We show that transcription factories in this tissue can be recognized as large nitrogen-rich structures with a mean diameter of 130 nm, which is considerably larger than that previously seen in transformed cultured cell lines. We show that KLF1-specialized factories are significantly larger, with the majority of measured factories occupying the upper 25th percentile of this distribution with an average diameter of 174 nm. In addition, we show that very highly transcribed genes associated with erythroid differentiation tend to occupy and share the largest factories with an average diameter of 198 nm. Our results suggest that individual factories are dynamically organized and able to respond to the increased transcriptional load imposed by multiple highly transcribed genes by significantly increasing in size.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.