ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2011
A high throughput, interactive imaging, bright-field wound healing assay.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- Engineering in vitro models to replicate the complexity of chronic wounds.Nature communications · 2026Review
- In vitro wound simulation: A high-throughput device for scratch assays.Methods (San Diego, Calif.) · 2026Article
- Methods to analyze cell migration data: fundamentals and practical guidelines.Nature methods · 2026Review
- A novel method for evaluating and visualizing scratch wound healing assays using level-set and image sector analysis.PNAS nexus · 2025Article
- In Vitro Wound Simulation: A High-Throughput Device for Scratch Assays.bioRxiv : the preprint server for biology · 2025Article
- Epithelial-to-mesenchymal transition (EMT) and cancer metastasis: the status quo of methods and experimental models 2025.Molecular cancer · 2025Review
- A programmable, open-source robot that scratches cultured tissues to investigate cell migration, healing, and tissue sculpting.Cell reports methods · 2024Article
- SCRATCH: A programmable, open-hardware, benchtop robot that automatically scratches cultured tissues to investigate cell migration, healing, and tissue sculpting.bioRxiv : the preprint server for biology · 2024Article
- An automated in vitro wound healing microscopy image analysis approach utilizing U-net-based deep learning methodology.BMC medical imaging · 2024Article
- Cell Migration Assays and Their Application to Wound Healing Assays-A Critical Review.Micromachines · 2024Review
- Chronic Wound Healing Models.ACS pharmacology & translational science · 2023Article
- Skin models of cutaneous toxicity, transdermal transport and wound repair.Burns & trauma · 2023Review
- In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings.Pharmaceutics · 2022Review
- Bioengineered Wound Healing Skin Models: The Role of Immune Response and Endogenous ECM to Fully Replicate the Dynamic of Scar Tissue Formation In Vitro.Bioengineering (Basel, Switzerland) · 2022Review
- Extracellular Matrix Alignment Directs Provisional Matrix Assembly and Three Dimensional Fibrous Tissue Closure.Tissue engineering. Part A · 2021Article
- Natural Compounds as Target Biomolecules in Cellular Adhesion and Migration: From Biomolecular Stimulation to Label-Free Discovery and Bioactivity-Based Isolation.Biomedicines · 2021Review
- Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy.Asian journal of pharmaceutical sciences · 2021Article
- The Frequent Sampling of Wound Scratch Assay Reveals the "Opportunity" Window for Quantitative Evaluation of Cell Motility-Impeding Drugs.Frontiers in cell and developmental biology · 2021Article
- Nanotechnology-Based Medical Devices for the Treatment of Chronic Skin Lesions: From Research to the Clinic.Pharmaceutics · 2020Review
- A microfluidics-based wound-healing assay for studying the effects of shear stresses, wound widths, and chemicals on the wound-healing process.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The wound healing assay is a commonly used technique to measure cell motility and migration. Traditional methods of performing the wound healing assay suffer from low throughput and a lack of quantitative data analysis. We have developed a new method to perform a high-throughput wound healing assay that produces quantitative data using the LEAP™ instrument. The LEAP™ instrument is used to create reproducible wounds in each well of a 96-well plate by laser ablation. The LEAP™ then records bright field images of each well at several time points. A custom texture segmentation algorithm is used to determine the wound area of each well at each time point. This texture segmentation analysis can provide faster and more accurate image analysis than traditional methods. Experimental results show that reproducible wounds are created by laser ablation with a wound area that varies by less than 10%. This method was tested by confirming that neuregulin-2β increases the rate of wound healing by MCF7 cells in a dose dependent manner. This automated wound healing assay has greatly improved the speed and accuracy, making it a suitable high-throughput method for drug screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.