ArticleMicrobial cell factories2011
Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome.
Article in Microbial cell factories, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 116 citations in OpenAlex.
- Division of labour enables Yarrowia lipolytica synthetic consortia to achieve higher growth rates from starch.Microbial cell factories · 2026Review
- Creating a large designer cellulosome in yeast to boost ethanol production.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Single-cell analysis of yeast surface display for designer cellulosome applications using a fluorescent protein complex.Microbiology spectrum · 2025Article
- Advances in the Functionalization of Vaccine Delivery Systems: Innovative Strategies and Translational Perspectives.Pharmaceutics · 2025Review
- Review
- Improving cellulosic ethanol production by an engineered yeast consortium displaying a pentafunctional mini-cellulosome.FEMS yeast research · 2025Article
- A systematic discussion and comparison of the construction methods of synthetic microbial community.Synthetic and systems biotechnology · 2024Review
- Spontaneously established syntrophic yeast communities improve bioproduction.Nature chemical biology · 2023Article
- Current challenges in designer cellulosome engineering.Applied microbiology and biotechnology · 2023Review
- An accessory enzymatic system of cellulase for simultaneous saccharification and co-fermentation.Bioresources and bioprocessing · 2022Article
- Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation.Microbial cell factories · 2022Article
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Review
- Cell-surface engineering of yeasts for whole-cell biocatalysts.Bioprocess and biosystems engineering · 2021Review
- Metabolic modelling approaches for describing and engineering microbial communities.Computational and structural biotechnology journal · 2021Review
- Deep reinforcement learning for the control of microbial co-cultures in bioreactors.PLoS computational biology · 2020Article
- EngineeringBiotechnology for biofuels · 2020Article
- Advances in the development and application of microbial consortia for metabolic engineering.Metabolic engineering communications · 2019Review
- Development of novel surface display platforms for anchoring heterologous proteins in Saccharomyces cerevisiae.Microbial cell factories · 2019Article
- Efficient yeast surface-display of novel complex synthetic cellulosomes.Microbial cell factories · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production. Ideally, a recombinant microorganism, possessing the capability to utilize cellulose for simultaneous growth and ethanol production, is of great interest. We have reported recently the use of a yeast consortium for the functional presentation of a mini-cellulosome structure onto the yeast surface by exploiting the specific interaction of different cohesin-dockerin pairs. In this study, we engineered a yeast consortium capable of displaying a functional mini-cellulosome for the simultaneous growth and ethanol production on phosphoric acid swollen cellulose (PASC).
resultsA yeast consortium composed of four different populations was engineered to display a functional mini-cellulosome containing an endoglucanase, an exoglucanase and a β-glucosidase. The resulting consortium was demonstrated to utilize PASC for growth and ethanol production. The final ethanol production of 1.25 g/L corresponded to 87% of the theoretical value and was 3-fold higher than a similar yeast consortium secreting only the three cellulases. Quantitative PCR was used to enumerate the dynamics of each individual yeast population for the two consortia. Results indicated that the slight difference in cell growth cannot explain the 3-fold increase in PASC hydrolysis and ethanol production. Instead, the substantial increase in ethanol production is consistent with the reported synergistic effect on cellulose hydrolysis using the displayed mini-cellulosome.
conclusionsThis report represents a significant step towards the goal of cellulosic ethanol production. This engineered yeast consortium displaying a functional mini-cellulosome demonstrated not only the ability to grow on the released sugars from PASC but also a 3-fold higher ethanol production than a similar yeast consortium secreting only the three cellulases. The use of more complex cellulosomal structures may further improve the overall efficiency for ethanol production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.