ArticleVirology journal2011
Loop-mediated isothermal amplification for detection of porcine circovirus type 2.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Distinguished Loop-Mediated Isothermal Amplification Assay to Detect Porcine Epidemic Diarrhea Virus Genotypes I and II.Veterinary sciences · 2025Article
- Development and Evaluation of an Immunochromatographic Strip and a Magnetic Chemiluminescence Immunoassay for Detection ofVeterinary sciences · 2025Article
- Implementation of point-of-care platforms for rapid detection of porcine circovirus type 2.Journal of veterinary science · 2024Article
- Development of a recombinase-aided amplification method combined with lateral flow dipstick assay to detectVeterinary world · 2023Article
- Revisiting Porcine Circovirus Infection: Recent Insights and Its Significance in the Piggery Sector.Vaccines · 2023Review
- Development of Duplex LAMP Technique for Detection of Porcine Epidemic Diarrhea Virus (PEDV) and Porcine Circovirus Type 2 (PCV 2).Current issues in molecular biology · 2022Article
- Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2.Virusdisease · 2018Article
- Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens.Transboundary and emerging diseases · 2017Article
- Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2.BioMed research international · 2017Article
- Development of a loop-mediated isothermal amplification method to rapidly detect porcine circovirus genotypes 2a and 2b.Virology journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPorcine circovirus type 2 (PCV2) is the primary causative agent of the emerging swine disease known as postweaning multisystemic wasting syndrome (PMWS). Nowadays, polymerase chain reaction (PCR) is still the most widespread technique in pathogen detection. Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method developed in 2000, will possibly replace PCR in the field of detection. To establish a LAMP method for rapid detection of PCV2, two pairs of primers were designed specially from the open reading frame 2 (ORF2) sequences of PCV2. A LAMP method for rapid detection of PCV2 was established. To compare with PCR, sensitivity and specificity of LAMP were evaluated using the optimized reaction system. The LAMP products could be determined by agarose gel electrophoresis or adding SYBR Green I dye.
resultsThe amplification of LAMP could be obtained at 63°C for 60 min. The detection limit was nearly 1 copy of DNA plasmid, more sensitive than PCR. There was no cross-reaction with porcine circovirus type 1 (PCV1), porcine pseudorabies virus (PRV) and porcine parvovirus (PPV) under the same conditions.
conclusionsLAMP is an useful rapid detection method with high sensitivity and specificity for PCV2.
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