ArticlePloS one2011
Comparative proteomic analysis of differentially expressed proteins in the urine of reservoir hosts of leptospirosis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Meta-analysis to estimate the load of Leptospira excreted in urine: beyond rats as important sources of transmission in low-income rural communities.BMC research notes · 2017Pooled it
- Serum profiling of leptospirosis patients to investigate proteomic alterations.Journal of proteomics · 2012Trial
- Multi-omics analysis of human patient samples identifies key immune factors inMicrobiology spectrum · 2026Article
- Identification and characterization of small RNAs expressed by Leptospira borgpetersenii serovar Hardjo and their conservation in the genus Leptospira.BMC microbiology · 2026Article
- Cultivation of Leptospira interrogans Within Rat Peritoneal Dialysis Membrane Chambers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Development of leptospiral virulence-modifying protein detection assay: implications for pathogenesis and diagnostic test development.Microbiology spectrum · 2025Article
- Identification of equine mares as reservoir hosts for pathogenic species ofFrontiers in veterinary science · 2024Article
- Antigen-Specific Urinary Immunoglobulin in Reservoir Hosts of Leptospirosis.Veterinary sciences · 2021Article
- Bovine endometrial cells do not mount an inflammatory response toReproduction & fertility · 2021Article
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- Isolation and propagation of leptospires at 37 °C directly from the mammalian host.Scientific reports · 2020Article
- Investigating the Immunological and Biological Equilibrium of Reservoir Hosts and PathogenicFrontiers in microbiology · 2020Review
- Proteomic Analysis of Urine from California Sea Lions ( Zalophus californianus): A Resource for Urinary Biomarker Discovery.Journal of proteome research · 2018Article
- Leptospiral 3-hydroxyacyl-CoA dehydrogenase as an early urinary biomarker of leptospirosis.Heliyon · 2018Article
- Inbred Rats as a Model to Study Persistent Renal Leptospirosis and Associated Cellular Immune Responsiveness.Frontiers in cellular and infection microbiology · 2018Article
- Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis.Scientific reports · 2017Article
- Reverse Vaccinology: An Approach for Identifying Leptospiral Vaccine Candidates.International journal of molecular sciences · 2017Review
- Pathogenic Leptospires Modulate Protein Expression and Post-translational Modifications in Response to Mammalian Host Signals.Frontiers in cellular and infection microbiology · 2017Article
- Proteomic analysis of urine exosomes reveals renal tubule response to leptospiral colonization in experimentally infected rats.PLoS neglected tropical diseases · 2015Article
- Post-translational modification of LipL32 during Leptospira interrogans infection.PLoS neglected tropical diseases · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rattus norvegicus is a natural reservoir host for pathogenic species of Leptospira. Experimentally infected rats remain clinically normal, yet persistently excrete large numbers of leptospires from colonized renal tubules via urine, despite a specific host immune response. Whilst persistent renal colonization and shedding is facilitated in part by differential antigen expression by leptospires to evade host immune responses, there is limited understanding of kidney and urinary proteins expressed by the host that facilitates such biological equilibrium. Urine pellets were collected from experimentally infected rats shedding leptospires and compared to urine from non-infected controls spiked with in vitro cultivated leptospires for analysis by 2-D DIGE. Differentially expressed host proteins include membrane metallo endopeptidase, napsin A aspartic peptidase, vacuolar H+ATPase, kidney aminopeptidase and immunoglobulin G and A. Loa22, a virulence factor of Leptospira, as well as the GroEL, were increased in leptospires excreted in urine compared to in vitro cultivated leptospires. Urinary IgG from infected rats was specific for leptospires. Results confirm differential protein expression by both host and pathogen during chronic disease and include markers of kidney function and immunoglobulin which are potential biomarkers of infection.
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