ArticleGenetics2012
Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- The kinase SPE-55 promotes localized assembly of the major sperm protein in Caenorhabditis elegans spermatocytes.Nature communications · 2026Article
- SPE-55 and VHA-18 are essential for FB-MO complex assembly and transport during spermatogenesis in C. elegans.Nature communications · 2026Article
- SPSP-1 is a sperm protein required for C. elegans hermaphrodite sperm production and embryogenesis.Biology open · 2026Article
- Inhibitor-2 directs formation of PP1 holoenzymes through a docking motif-dependent transfer of catalytic subunits to adapters.bioRxiv : the preprint server for biology · 2026Article
- A sperm-oocyte protein partnership required for egg activation in Caenorhabditis elegans.Development (Cambridge, England) · 2025Article
- Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells.Cell discovery · 2025Article
- C. elegans spermatocyte divisions show a weak spindle checkpoint response.Journal of cell science · 2024Article
- RNAi-dependent expression of sperm genes in ADL chemosensory neurons is required for olfactory responses inFrontiers in molecular biosciences · 2024Article
- C. elegans germ granules sculpt both germline and somatic RNAome.Nature communications · 2023Article
- Spermiogenesis inBiomolecules · 2023Review
- NHR-23 and SPE-44 regulate distinct sets of genes during Caenorhabditis elegans spermatogenesis.G3 (Bethesda, Md.) · 2022Article
- The Caenorhabditis elegans ASPP homolog APE-1 is a junctional protein phosphatase 1 modulator.Genetics · 2022Article
- Subcellular patterns of SPE-6 localization reveal unexpected complexities in Caenorhabditis elegans sperm activation and sperm function.G3 (Bethesda, Md.) · 2021Article
- Transcriptomic insights into the effects of CytCo, a novel nematotoxic protein, on the pine wood nematode Bursaphelenchus xylophilus.BMC genomics · 2021Article
- The intrinsically disordered protein SPE-18 promotes localized assembly of MSP inDevelopment (Cambridge, England) · 2021Article
- The molecular underpinnings of fertility: Genetic approaches inAdvanced genetics (Hoboken, N.J.) · 2021Article
- Spatial Transcriptomics of Nematodes Identifies Sperm Cells as a Source of Genomic Novelty and Rapid Evolution.Molecular biology and evolution · 2021Article
- Identification and functional analysis of five genes that encode distinct isoforms of protein phosphatase 1 in Nilaparvata lugens.Scientific reports · 2020Article
- Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract.Journal of visualized experiments : JoVE · 2019Article
- The meiotic phosphatase GSP-2/PP1 promotes germline immortality and small RNA-mediated genome silencing.PLoS genetics · 2019Article
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Authors and funding
9 authors.
Funding
Abstract
Sperm from different species have evolved distinctive motility structures, including tubulin-based flagella in mammals and major sperm protein (MSP)-based pseudopods in nematodes. Despite such divergence, we show that sperm-specific PP1 phosphatases, which are required for male fertility in mouse, function in multiple processes in the development and motility of Caenorhabditis elegans amoeboid sperm. We used live-imaging analysis to show the PP1 phosphatases GSP-3 and GSP-4 (GSP-3/4) are required to partition chromosomes during sperm meiosis. Postmeiosis, tracking fluorescently labeled sperm revealed that both male and hermaphrodite sperm lacking GSP-3/4 are immotile. Genetic and in vitro activation assays show lack of GSP-3/4 causes defects in pseudopod development and the rate of pseudopodial treadmilling. Further, GSP-3/4 are required for the localization dynamics of MSP. GSP-3/4 shift localization in concert with MSP from fibrous bodies that sequester MSP at the base of the pseudopod, where directed MSP disassembly facilitates pseudopod contraction. Consistent with a role for GSP-3/4 as a spatial regulator of MSP disassembly, MSP is mislocalized in sperm lacking GSP-3/4. Although a requirement for PP1 phosphatases in nematode and mammalian sperm suggests evolutionary conservation, we show PP1s have independently evolved sperm-specific paralogs in separate lineages. Thus PP1 phosphatases are highly adaptable and employed across a broad range of sexually reproducing species to regulate male fertility.
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