ArticleCellular oncology (Dordrecht, Netherlands)2011
Cervical carcinoma-associated fibroblasts are DNA diploid and do not show evidence for somatic genetic alterations.
Article in Cellular oncology (Dordrecht, Netherlands), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 26 citations in OpenAlex.
- Exosomes derived from colorectal cancer cells take part in activation of stromal fibroblasts through regulating PHLPP isoforms.EXCLI journal · 2024Article
- A clinically applicable molecular classification of oncocytic cell thyroid nodules.Endocrine-related cancer · 2023Article
- "Pulsed Hypoxia" Gradually Reprograms Breast Cancer Fibroblasts into Pro-Tumorigenic Cells via Mesenchymal-Epithelial Transition.International journal of molecular sciences · 2023Article
- Karyotype Aberrations in Action: The Evolution of Cancer Genomes and the Tumor Microenvironment.Genes · 2021Review
- Review
- The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy.Cancers · 2019Review
- Cytoskeletal alteration modulates cancer cell invasion through RhoA-YAP signaling in stromal fibroblasts.PloS one · 2019Article
- Review
- Cancer-associated fibroblasts support vascular growth through mechanical force.Scientific reports · 2017Article
- Genetic alterations and epigenetic alterations of cancer-associated fibroblasts.Oncology letters · 2017Article
- Epigenetic control of the tumor microenvironment.Epigenomics · 2016Review
- Carcinoma-associated fibroblasts: orchestrating the composition of malignancy.Genes & development · 2016Review
- Digital Sorting of Pure Cell Populations Enables Unambiguous Genetic Analysis of Heterogeneous Formalin-Fixed Paraffin-Embedded Tumors by Next Generation Sequencing.Scientific reports · 2016Article
- Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival.Oncotarget · 2015Article
- [Advancement of phenotype transformation of cancer-associated fibroblasts: from genetic alterations to epigenetic modification].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2015Review
- The genomic and transcriptomic landscape of a HeLa cell line.G3 (Bethesda, Md.) · 2013Article
- Multiparameter DNA content analysis identifies distinct groups in primary breast cancer.British journal of cancer · 2013Article
- Loss of heterozygosity and copy number alterations in flow-sorted bulky cervical cancer.PloS one · 2013Article
- Article
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4 authors at 1 institution in 1 country.
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Abstract
backgroundCancer-associated fibroblasts (CAFs) have been recognized as important contributors to cancer development and progression. However, opposing evidence has been published whether CAFs, in addition to epigenetic, also undergo somatic genetic alterations and whether these changes contribute to carcinogenesis and tumour progression.
methodsWe combined multiparameter DNA flow cytometry, flow-sorting and 6K SNP-arrays to study DNA aneuploidy, % S-phase, loss of heterozygosity (LOH) and copy number alterations (CNAs) in cervical cancer-associated stromal cell fractions (n = 57) from formalin-fixed, paraffin-embedded (FFPE) samples. Tissue sections were examined for the presence of CAFs. Microsatellite analysis was used to confirm LOH findings.
resultsSmooth muscle actin and vimentin immunohistochemistry verified the presence of CAFs in all cases tested. However, we found no evidence for DNA aneuploidy, somatic genetic alterations in the vimentin-positive stromal cell fractions of any samples, while high frequencies of DNA content abnormalities (43/57) and substantial numbers of CNAs and LOH were identified in the keratin-positive epithelial cell fractions. LOH hot-spots on chromosomes 3p, 4p and 6p found were confirmed by microsatellite analysis.
conclusionFrom our study we conclude that stromal cell fractions from cervical carcinomas are DNA diploid, have a genotype undistinguishable from patient-matched normal tissue and are genetically stable. Using flow cytometry and SNP-arrays, stromal genetic changes do not seem to play a role during cervical carcinogenesis and progression. In addition, the stromal cell fraction of cervical carcinomas can be used as reference allowing large retrospective studies of archival FFPE tissues for which no normal reference tissue is available.
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